文献类型：期刊文献

中文题名：改性凹凸棒黏土/羧甲基壳聚糖协同稳定桉叶精油Pickering乳液制备及缓释抑菌性能

英文题名：Preparation and Sustained-release Antibacterial Activity of Eucalyptus Leaf Essential Oil Pickering Emulsion Stabilized by Modified Attapulgite Nanoparticles and Carboxymethyl Chitosan

作者：史沛青[1];郑艳茹[1];陈晖[1,2];王轩栋[1];韩明明[1];施小宁[1,3]

第一作者：史沛青

机构：[1]甘肃中医药大学药学院,甘肃兰州730000;[2]甘肃中医药大学理化实验中心,甘肃兰州730000;[3]甘肃省中医药研究中心,甘肃兰州730000

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

年份：2022

卷号：43

期号：18

起止页码：47

中文期刊名：食品科学

外文期刊名：Food Science

收录：CSTPCD;;EI(收录号：20224313006711);Scopus;北大核心:【北大核心2020】；CSCD:【CSCD2021_2022】；

基金：甘肃省自然科学基金项目(20JR10RA328);甘肃省教育科技创新项目(2022A-066);甘肃省中医药研究中心开放课题(zyzx-2020-29)。

语种：中文

中文关键词：改性凹凸棒黏土;羧甲基壳聚糖;桉叶精油;Pickering乳液;抑菌活性

外文关键词：modified attapulgite;carboxymethyl chitosan;eucalyptus essential oil;Pickering emulsion;antibacterial activity

摘要：以油酸钠表面改性的凹凸棒黏土(modified-attapulgite,M-APT)与羧甲基壳聚糖(carboxy-methyl chitosan,CMCS)协同稳定制备桉叶精油Pickering乳液,通过乳液静置乳析、乳滴显微形貌及流变学性质,考察M-APT、CMCS质量分数及桉叶精油体积分数对乳液稳定性影响。结果表明,油酸钠改性使APT接触角θ从15.2°增大到83.7°,可不可逆吸附于油滴表面,乳滴粒径随M-APT质量分数增大而减小;亲水CMCS链自组装包封表面吸附M-APT粒子的油滴。乳液储存模量(G’)随CMCS质量分数增加明显增大,乳液稳定性增强。琼脂盘打孔法,对比考察纯桉叶精油和乳液对金黄色葡萄球菌和枯草芽孢杆菌的抑菌活性,发现乳液体系抑菌圈直径均略大于纯精油体系,这主要是因为M-APT吸附在油滴表面实现了对精油的包封,降低了其挥发性。菌类生长动力学实验也证实了这一结果,即M-APT/CMCS协同作用可实现对精油的包封稳定,提高其抑菌活性和实现长效缓释抑菌,这对拓展植物精油在生物抑菌剂方面的应用具有重要的参考价值。
For the purpose of enhancing the stability and antibacterial activity of eucalyptus leaf essential oil,a eucalyptus oil Pickering emulsion was fabricated using a mixture of sodium oleate modified attapulgite nanoparticles(M-APT)and carboxymethyl chitosan(CMCS)as a stabilizer.The effects of M-APT,CMCS and essential oil concentration on the emulsion’s stability were evaluated in terms of creaming behavior,micromorphology and rheological properties.The results showed that the modification of APT with sodium oleate increased its contact angles(θ)from 15.2°to 83.7°,which facilitated irreversible adsorption of M-APT nanoparticles onto the surface of oil droplets.The droplet diameter of the emulsion decreased with an increase in M-APT concentration.The hydrophilic CMCS chains were self-assembled to form a 3D network,which encapsulated the M-APT-adsorbed oil droplets.The storage modulus(G’)of the emulsion increased with increasing CMCS concentration,and so did the stability against creaming.Furthermore,the diameters of inhibition zone of the Pickering emulsion against Staphylococcus aureus and Bacillus subtillis were slightly larger than those of eucalyptus essential oil as determined by the agar diffusion method.This could be attributed to the fact that M-APT adsorption onto the oil droplet surface allows for encapsulation of the essential oil,reducing its volatility.The results of bacterial growth kinetics proved the synergistic effect of M-APT and CMCS in encapsulating the essential oil for improved stability and sustained-released antibacterial activity,which is great significance to expand the application of plant essential oil as a biological antibacterial agent.