文献类型：期刊文献

中文题名：苍术-白术药对治疗小儿厌食症的机制研究

英文题名：Exploring the Mechanism of Cangzhu-Baizhu in the Treatment of Infantile Anorexia based on Network Pharmacology

作者：吕彩兰[1];杨楠[1];陈静[1];李玉霞[1]

第一作者：吕彩兰

机构：[1]甘肃中医药大学附属医院,甘肃兰州730020

第一机构：甘肃中医药大学第二附属医院

年份：2022

卷号：34

期号：1

起止页码：76

中文期刊名：中医药临床杂志

外文期刊名：Clinical Journal of Traditional Chinese Medicine

基金：国家自然科学基金青年项目(编号:81603006);甘肃省中医药管理局项目(编号:GZK201932)。

语种：中文

中文关键词：苍术-白术;小儿厌食症;网络药理学

外文关键词：Cangzhu-Baizhu;Anorexia in children;Network pharmacology

摘要：目的:基于网络药理学方法探究苍术-白术药对治疗小儿厌食症(IA)的作用。方法:在TCMSP数据库以口服生物利用度(OB)≥30%、类药性(DL)≥0.18 为条件筛选苍术-白术药对的有效活性成分,并预测其靶点,采用UniProt数据库对靶点校正;在GeneCards数据库检索IA靶点;利用R语言软件筛选药物与IA的共同靶点,利用Cytoscape3.8.3软件构建苍术-白术药对的有效活性成分-疾病靶点互作网络,在SRTING11.0数据库构建共同靶点蛋白互作网络(PPI),并利用R语言软件对PPI结果可视化分析;利用R语言软件对药物与疾病共同靶点进行基因本体(Gene Ontology,GO)分析和京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)通路富集分析。结果:检索苍术-白术药对有效活性成分5个、活性成分靶点37个、IA靶点920个,筛选药物活性成分与疾病共同靶点15个,PPI发现互作频次较高共同靶点蛋白有周期蛋白1、半胱氨酸天冬氨酸特异性蛋白酶3、白介素-6糖原合酶激酶3β骨髓细胞白血病1半胱氨酸天冬氨酸特异性蛋白酶-9等;GO分析发现共同靶点参与的生物学过程包括对类固醇激素的反应、缺乏配体的信号转导、在没有配体的情况下的外在凋亡信号通路、外在凋亡信号通路、对糖皮质激素的反应、对皮质类固醇的反应、神经元死亡等834个;KEGG 富集分析结果发现共同靶点富集参与的通路包括麻疹、p53信号通路、大肠癌、前列腺癌、脂质和动脉粥样硬化、卡波西氏肉瘤相关疱疹病毒感染、小细胞肺癌、PI3K-Akt信号通路、EB病毒感染、凋亡-多种等71条通路。结论:苍术-白术药对可能是通过作用于相关通路与关键靶点蛋白,通过调节肠道菌群、改善肠道动力、修复胃肠道黏膜损及抗炎等机制防治IA。
Objective:To explore the effect of Cangzhu-Baizhu on the treatment of infantile anorexia (IA) based on the method of network pharmacology.Methods:In the TCMSP database,the effective active ingredients of the Cangzhu-Baizhu drug pair were screened based on the conditions of oral bioavailability (OB) ≥ 30% and drug-like properties (DL) ≥ 0.18,and their targets were predicted.UniProt database was used to correct the targets.;Search for IA targets in the GeneCards database;use R language software to screen the common targets of drugs and IA,and use Cytoscape 3.8.3 software to construct the effective active ingredient-disease target interaction network of the Cangzhu-Baizhu drug pair,in the SRTING11.0 database Construct a common target protein interaction network (PPI),and use R language software to visually analyze PPI results;use R language software to perform Gene Ontology (GO) analysis on common targets of drugs and diseases and Kyoto Gene and Genome Encyclopedia Encyclopedia (Kyoto Encyclopedia of Genes and Genomes,KEGG) pathway enrichment analysis.Results:Retrieved 5 effective active ingredients,37 active ingredient targets,and 920 IA targets for Cangzhu-Baizhu medicine.15 common targets for active ingredients and diseases were screened.PPI found that the interaction frequency was higher and the common target protein was higher.Cyclin 1,cysteine aspartate specific protease 3,interleukin-6 glycogen synthase kinase 3β,myeloid leukemia 1 cysteine aspartate specific protease-9,etc.;GO analysis found common The biological processes involved in the target include the response to steroid hormones,the lack of ligand signal transduction,the extrinsic apoptosis signaling pathway in the absence of ligand,the extrinsic apoptosis signaling pathway,and the response to glucocorticoids,Response to corticosteroids,neuron death,etc.834;KEGG enrichment analysis found that the pathways involved in the enrichment of common targets include measles,p53 signaling pathway,colorectal cancer,prostate cancer,lipid and atherosclerosis,card Percy’s sarcoma-related herpes virus infection,small cell lung cancer,PI3K-Akt signaling pathway,Epstein-Barr virus infection,apoptosis-many other 71 pathways.Conclusion:Cangzhu-Baizhu drug pair may act on related pathways and key target proteins to prevent and treat IA by regulating the intestinal flora,improving intestinal motility,repairing gastrointestinal mucosal damage and anti-inflammatory mechanisms.