文献类型：期刊文献

英文题名：Harmine suppresses the malignant phenotypes of Lewis lung carcinoma cells by inhibiting EGFR phosphorylation

作者：Zhang, Xueliang[1,2];Wang, Tao[3];Hu, Yonghua[4,5];Fu, Caihong[6,7];Lian, Xiaowen[8];Li, Yan[9];Zhao, Ruoyu[1,2]

第一作者：Zhang, Xueliang

通信作者：Zhao, RY[1];Zhao, RY[2]

机构：[1]Sun Yat Sen Univ, Gansu Hosp, Canc Ctr, Dept Gastrointestinal Med oncol, Lanzhou 730050, Gansu, Peoples R China;[2]Gansu Prov Canc Hosp, Dept Gastrointestinal Oncol, Lanzhou 730050, Gansu, Peoples R China;[3]Gansu Prov Acad Inst Med Res, Res Ctr Translat Med, Lanzhou 730050, Gansu, Peoples R China;[4]Gansu Univ Chinese Med, Clin Med Coll 1, Lanzhou 730099, Gansu, Peoples R China;[5]Gansu Univ Chinese Med, Affiliated Hosp 2, Affiliated Hosp, Lanzhou 730099, Gansu, Peoples R China;[6]Gansu Prov Canc Hosp, Dept Thorac Med Oncol, Lanzhou 730050, Gansu, Peoples R China;[7]Sun Yat Sen Univ, Gansu Hosp, Canc Ctr, Dept Thorac Med oncol, Lanzhou 730050, Gansu, Peoples R China;[8]Gansu Prov Acad Inst Med Res, Dept Med Biotechnol, Lanzhou 730050, Gansu, Peoples R China;[9]Gansu Univ Chinese Med, Clin Med Coll 1, Lanzhou 730099, Gansu, Peoples R China

第一机构：Sun Yat Sen Univ, Gansu Hosp, Canc Ctr, Dept Gastrointestinal Med oncol, Lanzhou 730050, Gansu, Peoples R China

通信机构：[1]corresponding author), Sun Yat Sen Univ, Gansu Hosp, Canc Ctr, Dept Gastrointestinal Med oncol, Lanzhou 730050, Gansu, Peoples R China;[2]corresponding author), Gansu Prov Canc Hosp, Lanzhou 730050, Gansu, Peoples R China.

年份：2026

卷号：18

期号：8

起止页码：6483

外文期刊名：AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001863396000002)】；

基金：This work was supported by the following grants: 1. The Natural Science Foundation of Gansu Province [Grant Number 23JRRA1801]; 2. The Gansu Provincial Health and Medical Research Program [Grant Number GSWSKY-2020-04]; 3. The Key Research and Development Project of Gansu Province Science and Technology Plan (22YF7FA099); 4. The Lanzhou Municipal Science and Technology Programme (2022-3-39); 5. Healthcare Service and Security Enhancement Fund (10000015Z155080000004).

语种：英文

外文关键词：Harmine; Lewis lung carcinoma; EGFR; bioinformatics; molecular docking

摘要：Objective: To investigate whether harmine (HM) suppresses the malignant phenotypes of Lewis lung carcinoma (LLC) cells through an epidermal growth factor receptor (EGFR)-mediated mechanism. Methods: Possible targets of HM and LLC-related genes were obtained from public databases, and overlappingtargets were identified. A protein-protein interaction (PPI) network was constructed, followed by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses. Molecular docking was performed to predict the binding mode of HM with EGFR. The expression of phosphorylated EGFR (p-EGFR) was examined in LLC cells. In vitro assays were used to evaluate the effects of HM on cell viability, proliferation, colony formation, migration, invasion, and apoptosis. EGFR overexpression was further applied to assess whether EGFR was involved in the effects of HM. Results: A total of 121 overlappingtargets between HM and LLC were identified, and EGFR was recognized as a core hub target in the PPI network. Molecular docking predicted that HM could fit into the ATP-binding pocket of EGFR, with a binding energy below-7.0 kcal/mol. In LLC cells, HM showed selective cytotoxicity and significantly inhibited EGFR phosphorylation. HM also reduced cell proliferation, colony formation, migration, and invasion, while promoting apoptosis. These effects were partially reversed by EGFR overexpression. Conclusion: HM suppresses the malignant phenotypes of LLC cells, at least in part, by inhibiting EGFR phosphorylation and EGFR-mediated signaling.