文献类型：期刊文献

中文题名：红芪超滤物对人肝癌HepG2细胞辐射敏感性的影响

英文题名：Effects of the Ultra-filtration Extract Mixture from Hedysarum Polybotrys on Human Liver Cells HepG2 Radiosensitivity

作者：寇炜[1,2];李应东[1,2];刘凯[2];郭晓蓥[2];董玉梅[2]

第一作者：寇炜

机构：[1]兰州大学基础医学院,兰州730000;[2]甘肃中医学院中西医结合系,兰州730000

第一机构：兰州大学基础医学院,兰州730000

年份：2013

卷号：33

期号：2

起止页码：220

中文期刊名：中国中西医结合杂志

外文期刊名：Chinese Journal of Integrated Traditional and Western Medicine

收录：MEDLINE(收录号：23646478);CSTPCD;;Scopus(收录号：2-s2.0-84897967034);北大核心:【北大核心2011】；CSCD:【CSCD2013_2014】；PubMed;

基金：国家自然科学基金资助项目(No.81160478);甘肃省2009年科技重大专项(No.092NKDA017)

语种：中文

中文关键词：红芪超滤物;HepG2细胞;辐射增敏

外文关键词：the ultra-filtration extract mixture from Hedysarum Polybotrys;HepG2 cell;radiosensitivity;

摘要：目的观察红芪超滤物(UEMHP)对人肝癌HepG2细胞辐射增敏的作用,并探讨其可能机制。方法用CCK-8法检测UEMHP对HepG2细胞生长抑制的影响;克隆形成实验检测UEMHP对HepG2的辐射增敏作用;通过流式细胞仪检测HepG2细胞周期分布的变化和凋亡率;采用RT-PCR法检测Survivin mRNA表达。结果在5～50mg/L浓度范围内,UEMHP对HepG2细胞的生长抑制作用呈时间和剂量依赖性(P<0.05);加药辐射组与单纯辐射组比较,克隆存活曲线显示加药辐射组各剂量点的存活分数均低于单纯辐射组(P<0.05),UEMHP能抑制HepG2细胞克隆形成,对HepG2有辐射增敏作用;流式细胞仪检测显示UEMHP具有去G2/M周期阻滞效应,加药辐射组细胞凋亡率(21.42%±3.74%)高于对照组(5.35%±0.41%)、单纯药物组(10.36%±1.75%)和单纯辐射组(10.58%±2.01%,P<0.01);RT-PCR显示加药辐射组Survivin mRNA(0.31±0.02)表达低于对照组(0.82±0.06)和单纯辐射组(0.58±0.04),差异有统计学意义(P<0.01)。结论 UEMHP对人肝癌HepG2细胞具有辐射增敏作用,其辐射增敏机制可能通过下调SurvivinmRNA的表达与促进细胞凋亡有关。
ObjectiveTo investigate the effects of the ultra-filtration extract mixture from Hedysarum Polybotrys （UEMHP） on the radiosensitivity of HepG2 cells， and to explore its possible mechanisms. MethodsThe proliferation inhibition effects of UEMHP on HepG2 cells was detected by CCK-8 assay. The colony formation assay was used for the survival fraction （SF） analysis. The distribution of the cell cycle and the apoptosis rate were detected using flow cytometry （FCM）. The survivin mRNA expression level was detected using reverse transcription-PCR assay. ResultsThe inhibition of UEMHP on HepG2 cells was time-and dose-dependent at the concentration ranging between 5－50 mg/L （P〈0.05）. The parameters of the two curve for SF （P〈0.05） showed statistical difference between the irradiation group and the UEMHP irradiation group. UEMHP could inhibit the clone formation of HepG2 cells and enhance the radiosensitivity of HepG2 cells. The results of FCM showed that UEMHP could induce G2/M phase arrest. The apoptosis rate in the UEMHP irradiation group （21.42%±3.74%） was higher than that in the control group （5.35%±0.41%）， the only UEMHP group （10.36%±1.75%）， or the irradiation group （10.58%±2.01%） （P〈0.01）. RT-PCR showed that the survivin mRNA expression level was lower in the UEMHP irradiation group （0.31±0.02） than in the control group （0.82±0.06） and the irradiation group （0.58±0.04） respectively， showing statistical difference （P〈0.01）. ConclusionUEMHP can enhance the radiosensitivity of HepG2 cells， and its possible mechanisms might be correlated to down-regulating the survivin mRNA expression and promoting the apoptosis.