文献类型：期刊文献

中文题名：当归红芪超滤物对辐射致H9C2心肌细胞损伤的保护作用

英文题名：Protective effect of Radix Angelica Sinensis and Radix Hedysari ultrafiltration on radiation-induced injury of H9C2 cardiomyocytes

作者：王新强[1];蒋虎刚[1];赵信科[1,2];汪鸣[1];王艳平[1];赵晓彬[1];韩金晏[1];李应东[1,2]

第一作者：王新强

机构：[1]甘肃中医药大学中西医结合学院,甘肃兰州730000;[2]甘肃中医药大学附属医院心血管内科,甘肃兰州730000

第一机构：甘肃中医药大学中西医结合学院

年份：2021

卷号：37

期号：21

起止页码：2886

中文期刊名：中国临床药理学杂志

外文期刊名：The Chinese Journal of Clinical Pharmacology

收录：CSTPCD;;北大核心:【北大核心2020】；CSCD:【CSCD2021_2022】；

基金：国家自然科学基金资助项目(81860786);中医药防治重大疾病科研课题基金资助项目(ZGKZD-2018-02);甘肃中医药研究中心开放课题基金资助项目(zyzx-2020-zx9、zyzx-2020-zx5)。

语种：中文

中文关键词：当归红芪超滤物;放射性心肌损伤;心肌细胞凋亡

外文关键词：Radix Angelica Sinensis and Radix Hedysari ultrafiltration;radiation myocardial injury;myocardial cell apoptosis

摘要：目的观察当归红芪超滤物对辐射致H9C2心肌细胞损伤的保护作用。方法采用大鼠灌胃的方法提取含药血清。体外培养H9C2心肌细胞,辐射诱导H9C2心肌细胞为心肌细胞损伤模型。将细胞随机分为空白对照组(不干预)、阴性对照组(0 Gy X线照射+生理盐水0.5 mL)、模型组(6 Gy X线照射+生理盐水0.5 mL)、盐酸贝那普利组(6 Gy X线照射+盐酸贝那普利大鼠血清0.5 mL)、当归红芪超滤物组(6 Gy X线照射+当归红芪超滤物大鼠血清0.5 mL)、盐酸贝那普利+当归红芪超滤物组(6 Gy X线照射+盐酸贝那普利+当归红芪超滤物大鼠血清0.5 mL干预)。用酶联免疫吸附(ELISA)法检测肌钙蛋白T(cTnT)、肌钙蛋白Ⅰ(cTnⅠ)及C-反应蛋白(CRP)表达;用实时荧光定量逆转录聚合酶链反应(qRT-PCR)法和蛋白质印迹(Western blot)法检测H9C2心肌细胞中casepase-3、casepase-9及Bax-2 mRNA和蛋白的表达;用5-乙炔基-2′-脱氧尿嘧啶核苷(EdU)染色观察各组H9C2心肌细胞的阳性细胞率;用鬼笔环肽染色观察各组H9C2心肌细胞骨架的改变状况。结果空白对照组、阴性对照组、模型组、盐酸贝那普利组、当归红芪超滤物组、盐酸贝那普利+当归红芪超滤物组的cTnT表达量分别为(11.19±1.38),(11.62±2.79),(90.31±13.48),(37.72±9.35),(46.39±12.01),(31.89±8.07)ng·L^(-1),cTnⅠ表达量分别为(7.88±3.24),(6.56±1.92),(30.17±7.23),(15.81±4.06),(19.05±5.34),(11.38±3.42)ng·L^(-1),CRP表达量分别为(2.73±0.29),(3.76±0.75),(36.87±3.88),(15.04±1.43),(19.57±1.65),(11.32±2.34)μmol·L^(-1),EdU阳性细胞率分别为(31.08±2.55)%,(30.32±1.11)%,(18.10±2.13)%,(24.86±1.14)%,(24.01±3.48)%,(30.11±1.28)%。空白对照组、盐酸贝那普利组、当归红芪超滤物组、盐酸贝那普利+当归红芪超滤物组与模型组比较,差异均有统计学意义(均P<0.05)。结论当归红芪超滤物可能通过上调Bcl-2蛋白,下调caspase-3、caspase-9蛋白的表达,对H9C2心肌细胞凋亡具有一定的保护作用。
Objective To observe the protective effect of Radix Angelica Sinensis and Radix Hedysari ultrafiltration(RAS-RH)on radiation-induced H9 C2 cardiomyocyte damage.Methods The medicated serum was extracted by intragastric administration of rats.H9 C2 cardiomyocytes were cultured in vitro,and radiation-induced H9 C2 cardiomyocytes were cardiomyocyte injury model.Cells were randomly divided into blank control group(no intervention),negative control group(0 Gy X-ray irradiation+normal saline 0.5 m L),model group(6 Gy X-ray irradiation+normal saline 0.5 m L),benazepril hydrochloride group(6 Gy X-ray irradiation+benazepril hydrochloride rat serum 0.5 m L),RAS-RH group(6 Gy X ray irradiation+RAS-RH rat serum 0.5 m L),benazepril hydrochloride+RAS-RH group(6 Gy X-ray irradiation+benazepril hydrochloride+RAS-RH rat serum 0.5 m L intervention).After intervention with medicated serum or irradiation of H9 C2 cardiomyocytes,enzyme-linked immunosorbent assay(ELISA)was used to detect the expression of cardiac troponin T(cTnT),cardiac troponinⅠ(cTnⅠ)and C-reactive protein(CRP);real-time fluorescence quantification polymerase chain reaction(qRT-PCR)and Western blotting were used to detected the mRNA and protein expression of casepase-3,casepase-9 and Bax-2 in H9 C2 cardiomyocytes;the positive cells in each group of H9 C2 cardiomyocytes were observed by EdU staining;phalloidin staining was used to observe the changes of the H9 C2 myocardial cytoskeleton in each group.Results The cTnT expression levels of blank control group,negative control group,model group,benazepril hydrochloride group,RAS-RH group and benazepril hydrochloride+RAS-RH group were(11.19±1.38),(11.62±2.79),(90.31±13.48),(37.72±9.35),(46.39±12.01),(31.89±8.07)ng·L^(-1),cTnⅠexpression levels were(7.88±3.24),(6.56±1.92),(30.17±7.23),(15.81±4.06),(19.05±5.34),(11.38±3.42)ng·L^(-1),CRP expression levels are(2.73±0.29),(3.76±0.75),(36.87±3.88),(15.04±1.43),(19.57±1.65),(11.32±2.34)μmol·L^(-1),the EdU positive cell rates were(31.08±2.55)%,(30.32±1.11)%,(18.10±2.13)%,(24.86±1.14)%,(24.01±3.48)%,(30.11±1.28)%.There were statistically significant differences between blank control group,benazepril hydrochloride group,RAS-RH group,benazepril hydrochloride+RAS-RH group and model group(all P<0.05).Conclusion RAS-RH may have a certain protective effect on H9 C2 cardiomyocyte apoptosis by up-regulating Bcl-2 protein and down-regulating the expression of caspase-3 and caspase-9 protein.