文献类型：期刊文献

中文题名：固本增骨方对卵巢切除模型大鼠骨代谢和骨微结构的影响

英文题名：Effects of Guben Zenggu Decoction on Bone Metabolism and Bone Microstructure in Ovariectomized Rats

作者：董万涛[1];巩彦龙[2];宋敏[2];黄凯[2];董平[2,3];宋志靖[2];王凯[2];海云翔[2]

第一作者：董万涛

机构：[1]甘肃中医药大学附属医院关节外科,兰州730020;[2]甘肃中医药大学中医临床学院,兰州730000;[3]内蒙古医科大学中医学院,呼和浩特010010

第一机构：甘肃中医药大学第二附属医院

年份：2019

卷号：50

期号：5

起止页码：679

中文期刊名：四川大学学报（医学版）

外文期刊名：Journal of Sichuan University(Medical Sciences)

收录：CSTPCD;;Scopus;北大核心:【北大核心2017】；CSCD:【CSCD2019_2020】；PubMed;

基金：甘肃省自然科学基金(No.17JR5RA056、No.1506RJZA048);甘肃省中医药防治慢性疾病重点实验室开放基金项目(No.GSMBKY2015-07);甘肃省中医药管理局科研课题(No.GZK-2016-17);甘肃省中药现代制药工程研究院项目(No.YWW-2015049)资助

语种：中文

中文关键词：绝经后骨质疏松症;固本增骨方;骨代谢指标;骨微结构

外文关键词：Postmenopausal osteoporosis;Guben Zenggu Decoction;Bone metabolic markers;Bone microstructures

摘要：目的观察固本增骨方对卵巢切除大鼠骨代谢和骨微结构的影响,探讨固本增骨方防治绝经后骨质疏松症的作用机制。方法采用卵巢切除法建立大鼠骨质疏松模型,随机分为模型对照组,戊酸雌二醇组,固本增骨方高、中、低剂量组,选取同龄大鼠作为空白对照组。用药干预12周后,腹腔麻醉,心脏采血,应用酶联免疫吸附法检测大鼠血清雌二醇(E2)、骨钙素(BGP)、Ⅰ型前胶原羧基端交联端肽(PICP)、Ⅰ型胶原吡啶交联终肽(ICTP)、抗酒石酸酸性磷酸酶-5b(TRAP-5b)水平;采用微型CT影像系统,对各组大鼠右侧股骨标本进行骨组织计量学分析。结果与空白对照组比较,模型对照组大鼠E2、骨特异性碱性磷酸酶(BALP)含量及相对骨体积分数(BV/TV)、骨小梁厚度(Tb.Th)、骨小梁数量(Tb.N)、连接密度(Conn.D)均降低(P<0.05),BGP、ICTP、PICP、TRAP-5b及骨小梁分离度(Tb.Sp)、结构模型指数(SMI)明显升高(P<0.05);给予药物干预后,与模型对照组相比,戊酸雌二醇组和固本增骨方各组E2、BALP及BV/TV、Tb.Th、Tb.N、Conn.D均增高(P<0.05),BGP、ICTP、PICP、TRAP-5b水平及Tb.Sp、SMI均下降(P<0.05);且固本增骨方高剂量组优于固本增骨方低剂量组(P<0.05),呈量效依赖关系。结论固本增骨方可改善卵巢切除大鼠模型骨代谢及骨微结构,从而发挥绝经后骨质疏松症的防治作用。
Objective To test the effects of Guben Zenggu Decoction on bone metabolism and bone microstructure in ovariectomized rats for the purpose of preventing and treating postmenopausal osteoporosis.Methods Osteoporosis rat models were established by ovariectomy.The model rats were randomly divided into control,estradiol valerate treatment,and Guben Zenggu Decoction treatment groups with high,medium and low dosages.After 12 weeks of treatments,10 rats from each group were randomly selected for cardiac blood sampling after abdominal anesthesia.The serum levels of estradiol(E2),osteocalcin(BGP),carboxyterminal of typeⅠprocollagen(PICP),collagen typeⅠpyridine crosslinking peptide(ICTP)and acid tartaric acid phosphatase-5 b(TRAP-5 b)were determined by ELISA.Bone histomorphometric analysis was performed on the right femoral specimen of rats using micro-CT imaging.Results The models rats had lower levels of E2,bone alkaline phosphatase(BALP)and relative bone volume fraction(BV/TV),trabecular thickness(Tb.Th),number of trabeculae(Tb.N)and connectivity density(Conn.D),and higher levels of BGP,ICTP,PICP,TRAP-5 b and degree of trabecular separation(Tb.Sp),structural model index(SMI)than their normal counterparts(P<0.05).Estradiol valerate and Guben Zenggu Decoction treatments increased the levels of E2,BALP,BV/TV,Tb.Th,Tb.N,and Conn.D significantly(P<0.05).Higher doses of Guben Zenggu Decoction resulted in higher changes(P<0.05).Conclusion Guben Zenggu Decoction can improve bone metabolism and bone micro-structure in ovariectomized rats,thus playing a preventive and therapeutic role in postmenopausal osteoporosis.