文献类型：期刊文献

中文题名：逆流色谱技术分离蛋白质和多肽的研究进展

英文题名：Advances in Technology of Countercurrent Chromatography for Separation of Protein and Peptide

作者：王高红[1,2];黄新异[1];段文达[1,2];全凯军[1,2];王秉鹏[1,3];樊瑞娜[1,3];邸多隆[1]

第一作者：王高红

通信作者：Di, DL[1];Di, DL[2]

机构：[1]中国科学院兰州化学物理研究所,中国科学院西北特色植物资源化学重点实验室,甘肃省天然药物重点实验室,兰州730000;[2]中国科学院大学,北京100039;[3]甘肃中医药大学药学院,兰州730000

第一机构：中国科学院兰州化学物理研究所,中国科学院西北特色植物资源化学重点实验室,甘肃省天然药物重点实验室,兰州730000

通信机构：[1]corresponding author), Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Chem Northwestern Plant Resources, Lanzhou 730000, Peoples R China;[2]corresponding author), Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Nat Med Gansu Prov, Lanzhou 730000, Peoples R China.

年份：2016

卷号：44

期号：10

起止页码：1600

中文期刊名：分析化学

外文期刊名：Chinese Journal of Analytical Chemistry

收录：CSTPCD;;EI(收录号：20181304950063);Scopus(收录号：2-s2.0-85044330230);WOS:【SCI-EXPANDED(收录号:WOS:000385829900007)】；北大核心:【北大核心2014】；CSCD:【CSCD2015_2016】；

基金：This work was supported by the National Natural Science Foundation of China (No. 201175142, 21505144) and the Natural Science Foundation of Gansu Province, China (No. 1506RJZA283), the Science and Technology Project of Inner Mongolia: Research and Application of Key Technology for Industrialization of Preparation Polypeptide from Camel Blood, the Project of Alashan Institute: High Value Product Development of Camel Blood; the Science and Technology Project of Alashan Technology Bureau: Support of High Value Product Development of Camel Blood.

语种：中文

中文关键词：逆流色谱;蛋白质;多肽;综述

外文关键词：Countercurrent chromatography; Protein; Peptide; Review

摘要：逆流色谱是一种连续高效的液-液分配色谱技术,具有进样量大、无不可逆吸附、回收率高和能有效保护样品生物活性等优点,在蛋白质和多肽的分离中具有独特的优势。本文综述了近年来几种新型的逆流色谱技术在蛋白质和多肽分离中的研究进展,并对该领域的未来发展进行了展望。
Countercurrent chromatography is a kind of continuous and effective liquid-liquid partition chromatography with many unique characteristics such as large load capacity,no irreversible adsorption,high recovery rate,low risk of sample denaturation and so on,which has irreplaceable advantages in separation of protein and peptide. This review presents the advances of several kinds of new technology of countercurrent chromatography in the separation of protein and peptide. The developing prospect of this field is also discussed.