文献类型：期刊文献

中文题名：通乳散癖汤对乳腺增生病模型大鼠乳腺组织Bcl-2、PCNA表达的影响

英文题名：Effect of Tongru Sanpi decoction on expression of Bcl-2,PCNA of tissue of mammary glands in rats with hyperplasia of breast glands

作者：李朝平[1];路艳清[2]

第一作者：李朝平

机构：[1]甘肃中医学院妇产科教研室;[2]甘肃省平凉高等医学专科学校

第一机构：甘肃中医药大学

年份：2011

卷号：28

期号：1

起止页码：61

中文期刊名：天津中医药

外文期刊名：Tianjin Journal of Traditional Chinese Medicine

收录：CSTPCD

基金：甘肃省自然科学基金暨中青年科技基金资助项目(3ZS041-A25-067)

语种：中文

中文关键词：通乳散癖汤;乳腺增生病;大鼠

外文关键词：Tongru Sanpi decoction; hyperplasia of mammary glands; rats

摘要：[目的]检测抗凋亡基因(Bcl-2)、增殖细胞核抗原(PCNA)基因表达与乳腺增生病组织学的相关性,通过乳腺增生病癌变趋势的生物学指标研究探讨通乳散癖汤对乳腺增生病的干预治疗作用。[方法]采用苯甲酸雌二醇联合黄体酮腹腔注射诱导建立乳腺增生病大鼠模型,分别用通乳散癖汤高、中、低剂量和对照药乳癖消灌胃治疗。免疫组化检测各组大鼠乳腺组织标本中Bcl-2、PCNA的表达情况。[结果]通乳散癖汤能够调整实验大鼠乳腺组织Bcl-2、PCNA的表达水平。[结论]通乳散癖汤可能是通过抑制细胞增殖,诱导凋亡,从而实现抑制乳腺增生的作用。
[Objective] The aim of the present study was to detect correlation between gene expression of Bcl-2,PCNA and histology of hyperplasia of mammary glands and to explore the intervention therapy of Tongru Sanpitang on hyperplasia of mammary glands by studying the biological indicator of the cancer-changing tendency of hyperplasia of mammary gland.[Methods] Hyperplasia of mammary glands were induced in rats by intraperitonneal injection of Estradiol Benzoate and Progesterone.It was treated respectively by perfusion Tongru Sanpitang（high,middle and low doses） into stomach and in the control drug,Rupixiao was perfused into stomach.Expression of Bcl-2,PCNA in histological specimen of mammary glands in each group in rats was detected with IHC.[Result] Tongru Sanpi decoction could regulate the expression level of Bcl-2,PCNA of mammary glands in experimental rats.[Conclusions] Tongru Sanpi decoction can probably inhibit the hyperplasia of mammary glands by inhibiting cell proliferation and inducing cell apoptosis.