文献类型：期刊文献

英文题名：Effect of Yifei Jianpi Prescription on Lipopolysaccharide-induced Lung Immune Inflammatory Response in Rats Based on STAT1/IRF3 Pathway; [基于 STAT1/IRF3 信号通路探讨益肺健脾方对脂多糖诱导的大鼠肺部免疫炎症反应的影响]

作者：Yang H.; Yang Y.; Yang Y.; Zhu Z.; Ma Q.; Wu Y.; Li H.; Zhang X.; Liu X.

第一作者：Yang H.

机构：[1]Gansu Provincial Engineering Laboratory for the Creation of New Traditional Chinese Medicine （TCM）Products, Key Laboratory of Excavation and Innovative Transformation of TCM in Gansu Province, Gansu University of Chinese Medicine, Lanzhou, 730000, China;[2]Affiliated Hospital of Gansu University of Chinese Medicine, Lanzhou, 730000, China

第一机构：甘肃中医药大学

通信机构：[2]Affiliated Hospital of Gansu University of Chinese Medicine, Lanzhou, 730000, China;[1]Gansu Provincial Engineering Laboratory for the Creation of New Traditional Chinese Medicine （TCM）Products, Key Laboratory of Excavation and Innovative Transformation of TCM in Gansu Province, Gansu University of Chinese Medicine, Lanzhou, 730000, China|[10735]甘肃中医药大学;

年份：2025

卷号：31

期号：1

起止页码：146

外文期刊名：Chinese Journal of Experimental Traditional Medical Formulae

收录：Scopus(收录号：2-s2.0-85212955503)

语种：英文

外文关键词：activator of transcription protein 1/interferon regulatory factor 3（STAT1/IRF3）signaling pathway; acute lung injury; immune inflammatory response; signal transducer; Yifei Jianpi prescription

摘要：Objective： To observe the effect of Yifei Jianpi prescription on the of signal transducer and activator of transcription protein 1（STAT1）/interferon regulatory factor 3（IRF3）signaling pathway in a pneumonia model induced by lipopolysaccharide（LPS）and to explore the mechanism of Yifei Jianpi prescription in improving lung immune and inflammatory responses. Methods：Sixty male SPF SD rats were used in this study. Ten rats were randomly assigned to the normal control group，and the remaining 50 were instilled with LPS in the trachea to establish a pneumonia model. After successful modeling，the rats were randomly divided into the model group，dexamethasone group（0.5 mg·kg-1），and Yifei Jianpi prescription high-dose （12 mg·kg-1），medium-dose（6 mg·kg-1），and low-dose（3 mg·kg-1）groups，with 10 rats in each group. Treatment was administered once daily，and the normal control and model groups received the same volume of normal saline. After 14 days，flow cytometry was used to detect the classification of whole blood lymphocytes. Enzyme-linked immunosorbent assay（ELISA）was used to measure serum levels of immunoglobulin G（IgG），immunoglobulin A（IgA），immunoglobulin M（IgM），and the content of tumor necrosis factor-α（TNF-α），interleukin-8（IL-8），interleukin-6（IL-6），and interleukin-10（IL-10）in alveolar lavage fluid（BALF）. Hematoxylin-eosin（HE）staining was used to observe lung tissue pathology and score the damage. Thymus weight，spleen weight，and wet-to-dry weight ratio（W/D）were recorded. Real-time fluorescence quantitative polymerase chain reaction （Real-time PCR）was used to detect the mRNA expression of STAT1，IRF3，IL-6，and interferon-alpha（IFN-α）in lung tissues，while Western blot was performed to assess the protein expression of STAT1，IRF3，IL-6，and IFN-α. Results：Compared with the normal control group，the model group showed significantly increased proportion of B lymphocytes in peripheral blood，decreased proportions of NK cells and CD4+/CD8+（P<0.05，P<0.01），decreased serum levels of IgG and IgA，significantly increased IgM levels（P<0.01），significantly elevated content of TNF- α，IL-6，and IL-8 in BALF，and significantly decreased IL-10 levels （P<0.01）. Lung tissue damage was evident，with significant increases in thymus and spleen weights and a higher W/D ratio （P<0.01）. The mRNA and protein expression of STAT1，IRF3，IFN- α，and IL-6 in lung tissues was significantly upregulated （P<0.05，P<0.01）. Compared with the model group，the Yifei Jianpi prescription groups showed significantly reduced proportions of B lymphocytes in peripheral blood，increased proportions of NK cells and CD4+/CD8+ ratios（P<0.05，P<0.01），significantly increased serum levels of IgG and IgA，significantly decreased IgM levels（P<0.05，P<0.01），significantly reduced levels of TNF-α，IL-6，and IL-8 in BALF，and significantly increased IL-10 levels（P<0.01）. Lung tissue damage was alleviated，thymus and spleen weights were significantly reduced，and the W/D ratio was markedly decreased（P<0.01）. The mRNA and protein expression of STAT1，IRF3，IFN- α，and IL-6 in lung tissues was significantly downregulated（P<0.05，P<0.01）. Conclusion： Yifei Jianpi prescription can alleviate lung tissue damage and improve immune and inflammatory responses in LPS-induced pneumonia rats. The mechanism may be related to the inhibition of STAT1/IRF3 signaling pathway activation. ? 2025 China Academy of Chinese Medical Sciences Institute of Chinese Materia Medica. All rights reserved.