文献类型：期刊文献

中文题名：甘肃地区164例宫颈癌人乳头瘤病毒基因分型检测的研究

英文题名：Detection study of gene typing of human papillomavirus of 164 patients with cervical cancer in Gansu province

作者：冯淑娴[1];王勇[2]

第一作者：冯淑娴

机构：[1]兰州军区兰州总医院473临床部妇产科,甘肃兰州730070;[2]甘肃中医学院,甘肃兰州730000

第一机构：兰州军区兰州总医院473临床部妇产科,甘肃兰州730070

年份：2014

卷号：18

期号：9

起止页码：79

中文期刊名：实用临床医药杂志

外文期刊名：Journal of Clinical Medicine in Practice

收录：CSTPCD

基金：中国高校医学期刊临床专项资金(11321275)

语种：中文

中文关键词：人乳头瘤病毒;基因芯片;基因分型;宫颈癌

外文关键词：HPV;gene chip;gene typing;cervical cancer

摘要：目的检测甘肃地区女性人乳头瘤病毒(HPV)感染亚型,了解其分布情况,为本地区预防HPV感染和防治宫颈癌提供病毒学数据。方法采用导流杂交基因芯片技术,对164份宫颈癌和100份其他生殖道疾病样本进行HPV DNA分型检测。结果 164例宫颈癌患者中14种HPV亚型被检出,HPVDNA阳性率85.4%(140/164),其中10种高危型依次为HPV-16(68.3%,112/164),-52(6.1%,10/164),-58、-18、-33(3.7%、each,6/164),-59(2.4%,4/164)和-31、-45(1.2%、each,2/164)型,4种低危型为HPV-6(1.2%、2/164)、-11(4.9%,8/164)、-42(1.2%,2/164)和-CP8304(2.4%,4/164)型。100例其他生殖系统疾病患者中,9种HPV基因型被检测到,HPV DNA阳性率28.0%(28/100),包括8种高危型HPV-16(12%,12/100)、-52(6.0%,6/100)、-68(4 0%,4/100)、-31、-18、-39、-58、-59(2.0%、each,2/100)和1种低危型为HPV-11(8 0%,8/100)。宫颈癌组多重感染17.1%(28/164),其他生殖道疾病组多重感染6.0%(6/100)。结论甘肃地区宫颈癌以高危型HPV感染为主要因素,其中HPV-16、-52是最常见亚型。其他女性生殖道疾病与HPV感染有一定相关性,该检测方法可用于女性生殖道疾病HPV的检查,以确定感染亚型,指导临床治疗和随访。
Objective To study the distribution and the subtypes of human papillomavirus （HPV）infection of female patients with cervical cancer and /or other genital diseases in Gansu province,attempting to provide virological data for the prevention of HPV infection and treatment of cervical cancer.Methods A total of 164 specimens with cervical cancer and 100patients with oth-er genital diseases were detected by flow-through hybridization and gene chip.Results 14 HPV subtypes were detected in the 164 patients with cervical cancer （positive HPV DNA rate：85.4%）, in which the 10 HPV subtypes with high risk were HPV-16 （68.3%,112 /164）,-52 （6.1%,10 /164）,-58,-18,-33 （3.7% each 6 /164）,-59 （2.4%,4 /164）and-31,-45 （1.2% each,2 /164） while 4 HPVsubtypesoflowriskwereHPV-6 （1 .2 % ,2 /1 6 4 ）,-1 1 （4 .9 % ,8 /1 6 4 ）,-4 2 （1.2%,2 /164）and-CP8304 （2.4%,4 /164）.9 HPV subtypes were detected in 100 patients with other genital diseases （positive HPV DNA rate：28.0%）,in which 8 HPV subtypes with high risk were HPV-16 （12%,12 /100）,-52 （6.0%,6 /100）,-68 （4.0%,4 /100）,-31,-18,-39,-58, and-59 （2.0%each,2 /100）while 1 HPV subtype with low risk was HPV-11 （8.0%,8 /100）. Multiple infection rate of cervical cancer was 17.1%（28 /164）and multiple infection rate of genital tractwas6 .0 % （6 /1 0 0 ）.Conclusion High risk HPV subtypes infection is the dominant factor for cervical cancer in Gansu province ,in which HPV-1 6 and HPV-5 2 are the most common subtypes.There are certain correlation between HPV infection and other genital diseases.The flow-through hybridization and gene chip technique can be applied to detect HPV of female pa-tients with genital infection to confirm subtype genotypes of HPV and to guide clinic treatment and follow-up visits.