文献类型：期刊文献

中文题名：西黄丸对大鼠乳腺上皮细胞凋亡及ER、PR表达的影响

英文题名：Effect of Xihuang pills on apoptosis and expression of ER and PR in rat mammary epithelial cells

作者：刘景楠[1];赵敏[1];吴平安[1];韩宇[2];韩涛[1]

第一作者：刘景楠

机构：[1]甘肃中医药大学药学院中药学教研室,甘肃兰州730000;[2]华中科技大学生命科学与技术学院,湖北武汉430074

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

年份：2020

卷号：36

期号：5

起止页码：710

中文期刊名：中国药理学通报

外文期刊名：Chinese Pharmacological Bulletin

收录：CSTPCD;;Scopus;北大核心:【北大核心2017】；CSCD:【CSCD2019_2020】；

基金：国家自然科学地区基金项目(No 81560722)。

语种：中文

中文关键词：西黄丸;大鼠乳腺上皮细胞;凋亡;雌二醇;孕激素;Bax/Bcl-2

外文关键词：Xihuang pills;rat mammary epithelial cells;apoptosis;estradiol;progesterone;Bax/Bcl-2

摘要：目的研究西黄丸(xihuang pills)对雌二醇(estradiol,E2)、孕酮(progesterone,P)诱导的大鼠乳腺上皮细胞凋亡和雌、孕激素受体表达的影响,探讨其抗乳腺增生机制。方法采用不同浓度西黄丸含药血清处理E2、P诱导增殖后的大鼠乳腺上皮细胞,CCK-8法检测(24、48、72 h)细胞增殖情况;Annexin V-FITC/PI双染后结合流式细胞术检测细胞凋亡;免疫组化法检测Bax、Bcl-2光密度值;蛋白免疫印迹技术检测雌二醇受体α(ERα)、雌二醇受体β(ERβ)、孕激素受体(PR)蛋白表达量。结果西黄丸含药血清可明显抑制E2、P处理后的大鼠乳腺上皮细胞增殖(P<0.05),并诱导其发生凋亡,影响凋亡相关蛋白Bcl-2、Bax的表达(P<0.05),并有效抑制雌激素受体ERα、ERβ的表达。结论西黄丸通过调节相关凋亡蛋白诱导乳腺上皮细胞凋亡,并能调节乳腺组织雌孕激素分泌,影响乳腺的增生与复旧,对治疗乳腺增生有重要意义。
Aim To investigate the effect of Xihuang pills on estradiol(E2)and progesterone(P)-induced apoptosis of rat mammary epithelial cells and the expression of estrogen and progesterone receptors,and to explore its anti-mammary hyperplasia mechanism.Methods Rat mammary epithelial cells were treated with different concentrations of Xihuangpills containing serumafter E2,P induced proliferation.The cell proliferation was detected by CCK-8 method(24,48,72 h).The apoptosis was detected by flow cytometry after AnnexinV-FITC/PI double staining.The average optical density of Bax and Bcl-2 was detected by immunohistochemistry;Estradiol receptor alpha(ER-α),estradiol receptor beta(ER-β),progesterone receptor(PR)protein expression were detected by Western blot.Results The serum containing Xihuang pillssignificantly inhibitedthe proliferation of rat mammary epithelial cells after E2 and P treatment(P<0.05),and induced apoptosis,affecting the expression of apoptosis-related proteins Bcl-2 and Bax(P<0.05),and effectively inhibiting the expression of estrogen receptors ERα,ERβ.Conclusions Xihuang pills can induce the apoptosis of mammary epithelial cells by regulating related apoptotic proteins,and regulate the secretion of estrogen and progesterone in breast tissues,affecting the proliferation and rejuvenation of breast,which is of great significance for the treatment of breast hyperplasia.