文献类型：期刊文献

中文题名：蛇葡萄素钠协同卡铂体外诱导肺癌Lewis细胞凋亡研究

英文题名：Cytotoxic effect of ampelopsin sodium combined with carboplatin on Lewis lung adenocarcinoma cell line

作者：缪苗苗[1,2];吴勇杰[2];覃红[2];张艳霞[2,3]

第一作者：缪苗苗

机构：[1]兰州市第二人民医院,甘肃兰州730046;[2]兰州大学基础医学院药理学研究所,甘肃省新药临床前研究重点实验室,甘肃兰州730000;[3]甘肃中医药大学,甘肃兰州730000

第一机构：兰州市第二人民医院,甘肃兰州730046

年份：2016

卷号：21

期号：11

起止页码：1271

中文期刊名：中国临床药理学与治疗学

外文期刊名：Chinese Journal of Clinical Pharmacology and Therapeutics

收录：CSTPCD;;CSCD:【CSCD2015_2016】；

语种：中文

中文关键词：蛇葡萄素钠;卡铂;肺癌Lewis细胞;细胞凋亡

外文关键词：ampelopsin sodium ; carboplatin ;mouse Lewis lung cancer cells; apoptosis

摘要：目的：考察蛇葡萄素钠（AMP-Na）单用及与卡铂合用对肺癌Lewis细胞增殖的抑制作用,初步探讨AMP-Na抑瘤作用机制。方法：应用MTT法考察AMP-Na单用及与卡铂合用对肺癌Lewis细胞的细胞毒作用;使用流式细胞仪检测凋亡相关基因Bax以及Bcl-2表达。结果：体外细胞毒实验结果表明,AMP-Na在12.5～200.0μg/m L的浓度范围内对肺癌Lewis细胞的增殖具有浓度依赖性的抑制作用,其IC_（50）为63.1μg/m L;6.3、12.5、25.0、50.0μg/m L的AMP-Na与卡铂3.1～25.0μg/m L联合用药对卡铂抑制Lewis细胞的增殖作用具有协同效应;流式细胞仪检测结果显示,AMP-Na 12.5、25.0、50.0、100.0μg/m L单用及与卡铂25.0μg/m L联合应用处理细胞48h后,Bax的表达升高,Bcl-2的表达降低（P〈0.01）,Bcl-2/Bax比率降低。结论：蛇葡萄素钠单用对Lewis细胞的增殖具有一定的抑制作用,与卡铂合用,对Lewis细胞增殖具有协同抑制效应;蛇葡萄素钠可能是通过下调Bcl-2并上调Bax从而促进肿瘤细胞凋亡。
AIM ： effect and mechanism To investigate the cytotoxic of ampelopsin sodium （ AMP- Na） alone or combined with carboplatin on Lewis lung cancer cells. METHODS： MTT colorimetric method was used to investigate cytotoxic effect of AMP-Na alone or combined with carboplatin on Lew- is lung cancer cells. Flow cytometry（FCM） was used to analyze the cell apoptosis. RESULTS： Experimental study on cytotoxicity in vitro showed that the proliferation of Lewis lung cancer ceils was inhibited by AMP-Na alone in a concentration-dependent manner ranging from 12. 5-200.0 μg/mL and its IC50 was 63.1 μg/mL. Combined with carboplatin, AMP-Na of 6.3, 12.5,25.0 and 50.0μg/mL had synergistic inhibitory effect on the proliferation of Lewis cells. FCM results showed the ratio of Bcl-2/ Bax was decreased significantly in Lewis cells trea- ted with AMP-Na （ 12.5, 25.0, 50.0, 100.0 μg/ mL） or in combination with earboplatin （25 μg/ mL） for 48 hours. CONCLUSION ： AMP-Na de- creases Lewis cells survival rate in a concentration- dependent manner. Combined with carboplatin, AMP-Na has synergistic inhibitory effect on the pro- liferation of Lewis cells. The apoptosis inducing effect is partially mediated by Bcl-2/Bax in Lewis cells treated with AMP-Na alone or in combination with carboplatin.