文献类型：期刊文献

英文题名：VTA1 and breast cancer: a potential indicator for diagnostic and prognostic evaluation

作者：Wang, Yongfeng[1,2,6];Yang, Xinyue[3];Jiang, Xianglai[2];Guo, Jiayang[3];Yang, Hao[1];Yin, Siyang[4];Zhang, Zechen[4];Li, Donghai[5,7];Li, Sheng[1,6,7]

第一作者：Wang, Yongfeng

通信作者：Li, S[1];Li, DH[2];Li, S[3];Li, DH[4];Li, S[4]

机构：[1]Lanzhou Univ, Clin Med Coll 1, Lanzhou, Gansu, Peoples R China;[2]Gansu Prov Peoples Hosp, Dept Gastrointestinal Surg, Lanzhou, Gansu, Peoples R China;[3]Lanzhou Univ, Sch Stomatol, Lanzhou, Gansu, Peoples R China;[4]Lanzhou Univ, Sch Pharm, Lanzhou, Gansu, Peoples R China;[5]Lanzhou First Peoples Hosp, Dept Pathol, Lanzhou, Gansu, Peoples R China;[6]Lanzhou First Peoples Hosp, Dept Gen Surg, Lanzhou, Gansu, Peoples R China;[7]Gansu Univ Tradit Chinese Med, Clin Med Coll 2, Lanzhou, Peoples R China

第一机构：Lanzhou Univ, Clin Med Coll 1, Lanzhou, Gansu, Peoples R China

通信机构：[1]corresponding author), Lanzhou Univ, Clin Med Coll 1, Lanzhou, Gansu, Peoples R China;[2]corresponding author), Lanzhou First Peoples Hosp, Dept Pathol, Lanzhou, Gansu, Peoples R China;[3]corresponding author), Lanzhou First Peoples Hosp, Dept Gen Surg, Lanzhou, Gansu, Peoples R China;[4]corresponding author), Gansu Univ Tradit Chinese Med, Clin Med Coll 2, Lanzhou, Peoples R China.|[10735]甘肃中医药大学;

年份：2025

外文期刊名：ONCOLOGIE

收录：;Scopus(收录号：2-s2.0-105024419219);WOS:【SCI-EXPANDED(收录号:WOS:001614744600001)】；

基金：The databases referenced above have been instrumental to this work. The authors extend their sincere thanks to the Key Laboratory of Molecular Diagnostics and Precision Medicine for Surgical Oncology in Gansu.

语种：英文

外文关键词：breast cancer; vacuolar protein sorting-associated protein VTA1 homolog (VTA1); biomarker; the cancer genome atlas program (TCGA); immunohistochemistry

摘要：Objectives Vacuolar protein sorting-associated protein VTA1 homolog (VTA1) encodes a factor involved in multivesicular body trafficking. This investigation sought to determine if VTA1 might function as a diagnostic indicator, a standalone prognostic element, and a potential therapeutic target in breast cancer.Methods We performed a pan-cancer analysis of VTA1 expression using TCGA data from 33 cancer types and assessed immune infiltration with TIMER2.0. Breast cancer data from TCGA and Gene Expression Omnibus (GEO) database were collected to complete the difference analysis, clinical feature correlation, prognosis analysis, enrichment analysis, and tumor microenvironment score analysis of VTA1 in BRCA. The presence of VTA1 in BRCA cells was verified by Reverse Transcription Quantitative Polymerase Chain Reaction (RT-qPCR), Western Blot (WB), and Immunohistochemistry (IHC).Results VTA1 expression was markedly elevated in breast cancer. Elevated levels were significantly linked to worse overall survival (OS). (hazard ratio [HR] = 1.791, 95 % CI: 1.206-2.659, p<0.01) and recurrence-free survival (RFS), indicating that VTA1 is an independent prognostic factor. In the tumor immune microenvironment, high VTA1 expression correlated with increased infiltration of myeloid and resting immune cells but decreased effector/activated immune cells, suggesting poor prognosis.Conclusions VTA1 is a promising prognostic indicator for various cancers, particularly in breast cancer. The expression of VTA1 is markedly elevated in breast cancer tissues relative to the adjacent normal tissues. Clinical analyses further suggest that VTA1 functions as an independent indicator associated with unfavorable outcomes in breast cancer. These findings imply that VTA1 may serve as a promising biomarker for diagnosis, therapeutic guidance, and survival prediction.