文献类型：期刊文献

英文题名：Temperature-switchable deep eutectic solvent extraction of polysaccharides from wolfberry fruits: Process optimization, structure characterization, and antioxidant activity

作者：Cao, Yu[1,2,3];Wang, Han[2,3];Jian, Yinglin[1];Hao, Gaofeng[1,2,3];Di, Duolong[1,2,3,4];Liu, Jianfei[1,2,3,4]

第一作者：Cao, Yu

通信作者：Di, DL[1];Liu, JF[1]

机构：[1]Gansu Univ Tradit Chinese Med, Sch Pharm, 35 Dingxi East Rd, Lanzhou 730000, Gansu, Peoples R China;[2]Chinese Acad Sci, CAS Key Lab Chem Northwestern Plant Resources, 18 Tianshui Middle Rd, Lanzhou 730000, Gansu, Peoples R China;[3]Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Nat Med Gansu Prov, 18 Tianshui Middle Rd, Lanzhou 730000, Gansu, Peoples R China;[4]Chinese Acad Sci, Lanzhou Inst Chem Phys, Lanzhou 730000, Gansu, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), Chinese Acad Sci, Lanzhou Inst Chem Phys, Lanzhou 730000, Gansu, Peoples R China.

年份：2024

卷号：398

外文期刊名：JOURNAL OF MOLECULAR LIQUIDS

收录：;EI(收录号：20241015680767);Scopus(收录号：2-s2.0-85186464242);WOS:【SCI-EXPANDED(收录号:WOS:001199191000001)】；

基金：The authors acknowledge the grant from Youth Innovation Promotion Association CAS (2023442) , the Science and Technology Plan Project of Lanzhou City (2022-2-10) , Science Foundation of Gansu Province (No. 22JR5RA116) , and the Science and Technology program of Gansu Province (No. 20CX9FG238) . The authors also are grateful to anonymous reviewers for their comments and peer-review.

语种：英文

外文关键词：Wolfberry; Polysaccharides; Temperature-switchable deep eutectic solvent; Characterization; Antioxidant activity

摘要：Polysaccharides of wolfberry (LBPs) with complex structures are most commonly used in the food and pharmaceutical industries as biologically active phytochemicals. However, the structure and yield of LBPs vary according to the extraction method used, and selecting their extraction media remains difficult. A novel type of temperature-switchable deep eutectic solvent (TS-DES), consisting of L-menthol and phenyl salicylate, was designed to extract LBPs from wolfberry fruits. TS-DES was a recyclable extractant above the maximum critical solution temperature (UCST). The increased dispersibility of extraction solvents was imparted by combining them with a high-speed shear dispersion emulsification (HSDE) machine. TS-DES was in full contact with the cell walls of wolfberries, leading to the complete opening of the cell walls, resulting in the efficient extraction of LBPs. Compared with traditional extraction, the yield of LBPs-D reached 4.35%, which was higher than that of LBPs-W (1.78%). This study showed that LBPs-D were high molecular weight, highly branched polysaccharides with antioxidant activity. TS-DES was separated at a lower level than UCST for regeneration and reuse. After 5 cycles, TS-DES still had excellent recovery and extraction performance. This study provides valuable information for efficiently extracting structurally rich bioactive polysaccharides from plants using TS-DES.