文献类型：期刊文献

中文题名：浓缩归脾丸对苯中毒小鼠T细胞亚群、血清溶血素、粒-巨噬细胞集落刺激因子的影响

英文题名：Effects of inspissated Guipi Pill on T cell sunsets, serumal hemolysin and granulocyte colony stimulating factor of Benzene poisoning mice

作者：刘艳杰[1];刘立[1];王晶[1]

第一作者：刘艳杰

机构：[1]甘肃中医药大学

第一机构：甘肃中医药大学

年份：2016

卷号：31

期号：12

起止页码：5256

中文期刊名：中华中医药杂志

外文期刊名：China Journal of Traditional Chinese Medicine and Pharmacy

收录：CSTPCD;;北大核心:【北大核心2014】；CSCD:【CSCD_E2015_2016】；

基金：甘肃省2009年重大中医药科研项目(No.GZK2009-08)~~

语种：中文

中文关键词：归脾丸;苯中毒;免疫功能;血清溶血素;粒-巨噬细胞集落刺激因子

外文关键词：Guipi Pill;Benzolism;Immunologic function;Serumal hemolysin;Granulocyte colony stimulating factor

摘要：目的:观察归脾丸对苯中毒小鼠低下的T细胞亚群数,以及血清溶血素和血清中粒-巨噬细胞集落刺激因子(G-CSF)水平的改善作用。方法:72只周昆明小鼠,随机分成正常对照组、模型组、西药组和归脾丸高、中、低剂量组。将正常对照组外的5组小鼠置于人工模拟高苯浓度环境下复制苯中毒模型。造模后,正常对照组、模型组给予0.9%氯化钠溶液0.2m L/只灌胃;归脾丸高、中、低剂量组分别按8、4、2mg·kg-1·d-1灌胃;西药组给予利血生1.5mg·kg-1·d-1,鳖肝醇5mg·kg-1·d-1混悬液灌胃。采血并用全自动血细胞分析仪、流式细胞仪检测外周血,T细胞亚群的计数和血清溶血素、G-CSF的水平。结果:与模型组比较,归脾丸各组与西药组的CD4+、CD8+和CD4+/CD8+的表达均显著上升(P<0.05)。归脾丸大、中剂量组能提高鸡红细胞半数溶血时的吸光度值(P<0.05);与西药组比较,归脾丸各剂量组能升高G-CSF水平(P<0.05)。结论:归脾丸对苯中毒小鼠低下的T细胞亚群CD4+/CD8+数,以及血清溶血素和G-CSF水平具有明显的改善作用。
Objective： To observe the effects of Guipi Pills（GPP） on T cell sunsets, serumal hemolysin and granulocyte colony stimulating factor（G-CSF） of benzene poisoning mice. Methods： Seventy-two mice were randomly divided into 6 groups： the control group, the model group, the western medicine group, the large, middle and small dose GPP groups. All the mice except for the control group were exposed to manually simulated high concentrations of benzene to establish benzolism model. After that, mice from the model group and the control group were administered with normal saline by gavage 0.2m L per mice; mice from the large, middle and small dose GPP groups were administered with 8, 4, 2mg/kg GPP per day respectively by gavage; mice from the western medicine group were administered with leucogen（1.5mg/kg） and batyl alcohol（5.0mg/kg） by gavage per day. The levels of T cell sunsets, serumal hemolysin and G-CSF were detected by automatic blood cell analyzer and flow cytometry. Results： Compared with the model group, the expression of CD4＋, CD8＋ and CD4/CD8 were increased in three GPP groups and western medicine group, and the differences were significant statistically（P〈0.05）. The absorbance of chicken red blood cell half hemolysis was increased by large and middle dose GPP（P〈0.05）. There were statistically differences in the level of G-CSF between the all GPP groups and the western medicine group（P〈0.05）. Conclusion： GPP could improve T cell subsets CD4＋/CD8＋, serumal hemolysin and G-CSF of benzene poisoning mice.