文献类型：期刊文献

中文题名：西黄丸对大鼠卵巢相关激素及其受体的影响

英文题名：Effects of Xihuang Pill on ovarian hormones and its receptors in rats

作者：赵敏[1];连小龙[1];刘景楠[1];金戈[2];兰咏梅[3];韩涛[1,4]

第一作者：赵敏

机构：[1]甘肃中医药大学药学院,兰州730000;[2]甘肃中医药大学基础医学院,兰州730000;[3]西北民族大学医学院,兰州730030;[4]甘肃省中药药理与毒理学重点实验室,兰州730000

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

年份：2018

卷号：34

期号：24

起止页码：2844

中文期刊名：中国临床药理学杂志

外文期刊名：The Chinese Journal of Clinical Pharmacology

收录：CSTPCD;;北大核心:【北大核心2017】；CSCD:【CSCD2017_2018】；

基金：国家自然科学基金地区基金资助项目(81560722)

语种：中文

中文关键词：西黄丸;卵巢;雌二醇;孕激素;孕激素受体

外文关键词：Xihuang Pill; ovary; estradiol; progesterone; progesterone receptor

摘要：目的研究西黄丸对大鼠卵巢雌二醇(E_2)、孕激素(P)与孕激素受体(PR)的影响。方法按随机数表法将Wistar雌性未孕大鼠分为5组:空白组、对照组和高、中、低3个剂量实验组,每组10只。空白组灌胃等体积蒸馏水,对照组给予1.8 mg·kg^(-1)·d^(-1)他莫昔芬灌胃,高、中、低3个剂量实验组分别给予2.16,1.08,0.54 g·kg^(-1)·d^(-1)西黄丸灌胃,连续给药1个月。以酶联免疫法检测卵巢E_2、P含量;以免疫组化法检测卵巢PR受体表达量。结果空白组、对照组和高、中、低3个剂量实验组的E_2分别为(45.85±17.39),(19.45±6.41),(28.74±14.54),(29.82±11.57)和(44.11±8.25)ng·L^(-1);这5组的P分别为(259.67±12.31),(301.21±15.01),(296.47±14.04),(301.56±22.81)和(287.13±14.16)pmol·L^(-1);对照组和高、中2个剂量实验组与空白组比较,E_2水平差异均有统计学意义(均P<0.01);对照组和高、中、低3个剂量实验组与与空白组比较,P水平差异均有统计学意义(均P<0.01)。这5组的PR水平(光密度值)分别为(4.16±0.92)×10~3,(4.82±0.14)×10~3,(5.53±0.61)×10~3,(5.44±0.60)×10~3和(4.89±0.29)×10~3;高、中2个剂量实验组与空白组比较,差异均有统计学意义(均P<0.01)。结论西黄丸能降低大鼠卵巢中E_2的含量,增加P的含量,促进PR的表达,说明西黄丸能够对卵巢的功能产生干扰作用。
Objective To investigate the effects of Xihuang Pill on estradiol(E2) ,progestogen P) and progestogen receptor PR) in rat.Methods Wistar female rats were randomly divided into 5 groups: blankgroup,control group,and high,medium and low dose experimentalgroups,with 10 rats in each group. Each rat in the control group wasgiven 1. 8 mg·kg ^-1·d^-1 tamoxifen,each rat in the high,medium andlow dose groups was given Xihuang Pill according to 2. 16,1. 08,0. 54g·kg ^-1·d^-1,and the blank group was given distilled water of equalvolume. The serum levels of E2 and P were detected by enzyme linked immunosorbentassay. The expression levels of PR receptors in ovaries weredetected by immunohistochemical method. Results The E2 content inblank group,control group,and high,medium and low dose experimentalgroups were respectively 45. 85 ± 17. 39 ) ,19. 45 ± 6. 41 ) ,28. 74 ± 14. 54) ,29. 82 ± 11. 57 ) ,44. 11 ± 8. 25 ) ng·L ^-1 . Thedifference between the control group,high and medium dosage experimentalgroups with blank group had statistical significance all P < 0. 01) .The P content in the 5 groups were respectively 259. 67 ± 12. 31 ) ,301. 21 ± 15. 01) ,296. 47 ± 14. 04) ,301. 56 ± 22. 81) ,287. 13 ± 14. 16) pmol·L -1 ; the difference betweenthe control group,high,medium and low dosage experimental groups with blank group had statistical significanceallP < 0. 01) . The PR levels optical density values ) in the 5 groups were respectively 4. 16 ± 0. 92 ) × 10^3,4. 82 ± 0. 14) × 10^3,5. 53 ± 0. 61) × 10^3,5. 44 ± 0. 60) × 10^3,4. 89 ± 0. 29) × 10^3,the difference between highand medium dosage experimental groups with blank group had statistical significance all P < 0. 01) . ConclusionXihuang Pill can reduce the content of E2 and increase the content of P in the ovaries of rats,promote the expression ofPR,which shows that Xihuang Pill can interfere with the function of ovaries.