文献类型：期刊文献

中文题名：归芪白术方对放射性脾损伤大鼠的防护作用

英文题名：Protective effect of Guiqibaizhu Recipe on radiation-induced spleen injury in rats

作者：李洋洋[1];张苡铭[1];张利英[1];周婷[1];何进鹏[3];周谷城[1];牛帆[1];刘永琦[1,2]

第一作者：李洋洋

机构：[1]甘肃中医药大学甘肃省高校重大疾病分子医学与中医药防治研究省级重点实验室,甘肃兰州730000;[2]中国科学院近代物理研究所甘肃省空间辐射生物学重点实验室,甘肃兰州730000;[3]甘肃中医药大学敦煌医学与转化省部共建教育部重点实验室,甘肃兰州730000

第一机构：甘肃中医药大学科研实验中心（甘肃省中医药标准化技术委员会秘书处）

年份：2021

卷号：37

期号：13

起止页码：1705

中文期刊名：中国临床药理学杂志

外文期刊名：The Chinese Journal of Clinical Pharmacology

收录：CSTPCD;;北大核心:【北大核心2020】；CSCD:【CSCD2021_2022】；

基金：国家自然科学基金资助项目(81473457;81973595);甘肃省高等学校科研基金资助项目(2015A-095);西北中藏药协同创新中心基金资助项目(XBXT2015-1);甘肃省中药药理与毒理实验室开放基金资助项目(zyzx-2020-18);甘肃省高校重大疾病分子医学与中医药防治研究省级重点实验室开放基金资助项目(FZYX17-18-13);敦煌医学与转化教育部重点实验室开放基金资助项目(DHYX19-13)。

语种：中文

中文关键词：放射性脾损伤;归芪白术方;核因子κB p65;炎症反应;防护作用

外文关键词：radiation-induced spleen injury;Guiqibaizhu Recipe;nuclear factorκB p65;inflammatory response;protective effect

摘要：目的探讨中药复方归芪白术方对大鼠放射性脾损伤的防护作用机制。方法用随机数字表法将大鼠分为3组:正常组、模型组和实验组,每组13只。用6 Gy X线全身照射制作大鼠放射性脾损伤模型。造模前14 d,实验组给予归芪白术方8.4 g·kg-1灌胃,正常组和模型组给予等量的生理盐水灌胃,1次/天。在照射后3 d动物取材,苏木精-伊红染色法观察脾组织的形态变化,免疫组化检测脾组织中核因子κB p56(NF-κB p65)的表达,蛋白质印迹法检测脾组织肿瘤坏死因子受体-1(TNFR1)、核因子κB抑制物激酶β(IKKβ)和环氧合酶-2(COX-2)蛋白表达水平(平均光密度值)。结果模型组大鼠脾组织可见明显的炎性细胞浸润;实验组大鼠脾组织中炎性细胞浸润减少。正常组、模型组和实验组的病理学评分分别为(2.53±0.16),(0.62±0.08)和(1.65±0.11)分;这3组的NF-κB p65蛋白表达水平分别为0.17±0.01,0.26±0.03和0.19±0.01;这3组的TNFR1蛋白表达水平分别为0.15±0.01,0.39±0.01和0.21±0.01;这3组的IKKβ蛋白表达水平分别为0.69±0.01,0.82±0.01和0.68±0.01;这3组的COX-2蛋白表达水平分别为0.55±0.01,0.68±0.01和0.44±0.01。上述指标:模型组与正常组相比,差异均有统计学意义(均P<0.0001);实验组与模型组相比,差异均有统计学意义(均P<0.0001)。结论归芪白术方通过抑制炎症反应发挥对放射性脾损伤的防护作用。
Objective To explore the protective effect and mechanism of Guiqibaizhu Recipe,a traditional Chinese medicine compound,on radiation-induced spleen injury in rats.Methods Sprague-Dawley rats were randomly divided into three groups according to random number table:normal group,model group and experimental group,13 rats in each group.6 Gy X-ray whole irradiation was used to make a rat model of radiation-induced spleen injury.Fourteen days before modeling,the experimental group was given Guiqibaizhu Recipe 8.4 g·kg-1 by gavage,and the normal group and model group were given the same amount of normal saline by gavage,once a day.Three days after irradiation,the animals were collected,and the morphological changes of spleen tissue were observed by hematoxylin-eosin staining method.Immunohistochemical detection of nuclear factor kappa B p65(NF-κB p65)expression in spleen tissue.Western blotting was used to detect the expression levels(average optical density values)of tumor necrosis factor receptor-1(TNFR1),inhibitor of nuclear factor kappa-B kinasebeta(IKKβ)and cyclooxygenase-2(COX-2)proteins in spleen tissue.Results Obvious inflammatory cell infiltration can be seen in the spleen tissue of rats in the model group;inflammatory cell infiltration in the spleen tissue of the experimental group was reduced.The pathological scores of the normal group,model group and experimental group were(2.53±0.16),(0.62±0.08)and(1.65±0.11)point,respectively;the expression levels of NF-κB p65 protein in the three groups were 0.17±0.01,0.26±0.03 and 0.19±0.01,respectively;the expression of TNFR1 protein in the three groups were 0.15±0.01,0.39±0.01 and 0.21±0.01;the expression of IKKβprotein were0.69±0.01,0.82±0.01 and 0.68±0.01,respectively;the expression of COX-2 protein were 0.55±0.01,0.68±0.01 and 0.44±0.01,respectively.Comparison between model group and normal group,the difference of the factors were significant(all P<0.0001);comparison between experimental group and model group,the difference of the factors were significant(all P<0.0001).Conclusion Guiqibaizhu Recipe can protect radiation-induced spleen injury by inhibiting inflammation.