文献类型：期刊文献

中文题名：温阳益气法对肿瘤微环境中骨髓间充质干细胞炎性平衡及遗传稳定性的调控

英文题名：Regulatory effects of warming yang and invigorating qi treatment on the inflammatory balance and genetic stability of bone marrow mesenchymal stem cells under tumor microenvironment

作者：高卓越[1];刘永琦[1,2];何建新[1];伍志伟[1,2];骆亚莉[1];苏韫[1];张利英[1];张齐[1];武有明[1];周妮娜[1]

第一作者：高卓越

机构：[1]甘肃中医学院甘肃省高校重大疾病分子医学与中医药防治研究省级重点实验室,甘肃省兰州市730000;[2]甘肃中医学院甘肃省高校重大疾病分子医学与中医药防治研究省级重点实验室,甘肃省兰州市 730000;[3] 敦煌医学与转化省部共建教育部重点实验室,甘肃省兰州市730000

第一机构：甘肃中医药大学科研实验中心（甘肃省中医药标准化技术委员会秘书处）

年份：2015

卷号：19

期号：14

起止页码：2267

中文期刊名：中国组织工程研究

外文期刊名：Chinese Journal of Tissue Engineering Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2014】；

基金：甘肃省杰出青年基金项目“基于调控Wnt等信号通路研究当归预防肺癌微环境中MSCs恶性转化的作用(1308RJDA008)”;国家自然科学基金项目“从炎性信号通路失衡研究肺癌微环境中MSCs恶性转化的分子机制及黄芪调控作用研究(81360588)”;“黄芪对重离子辐射诱发肺癌相关MSCs旁效应及基因组不稳定的防护机制研究(81473457)”~~

语种：中文

中文关键词：干细胞;骨髓干细胞;炎性平衡;炎骨髓间充质干细胞;温阳益气;遗传稳定性;国家自然科学基金

外文关键词：Bone Marrow;Mesenchymal Stem Cells;Tumor Microenvironment;Drugs,Chinese Herbal

摘要：背景:骨髓间充质干细胞在癌性微环境中的遗传稳定性问题如何进一步明确并加以有效预防,日益成为推广其临床应用的关键问题。非可控性炎症和肿瘤之间存在互为因果现象,越来越受到科学界关注。目的:分析骨髓间质干细胞在肿瘤微环境中的炎性信号通路及遗传稳定性的变化,并基于中医学温阳固肾、益气升阳、扶正祛邪理论为指导,探讨温阳益气法提高骨髓间质干细胞在肿瘤相关炎性微环境中的效应及其作用机制。方法:以"骨髓间充质干细胞,炎性平衡,补肾,温阳,益气,升阳,肿瘤,癌,阳化气,阴成形,遗传稳定性"为中文检索词,以"bone marrow mesenchymal stem cells,inflammatory balance,replenishing qi warming yang,strengthening shen,elevate yang,tumor,cancer,yang can transform qi,yin shaping up body,genetic stability"为英文检索词,应用计算机检索中国知网CNKI数据库、维普和PubM ed数据库,严格按纳入标准、排除标准进行文献质量评估和筛选,最终选择42篇文献进行综合分析。结果与结论:在理化因素诱导或肿瘤微环境中,骨髓间充质干细胞可促进肿瘤增殖、侵袭转移,另外骨髓间充质干细胞自身也可被诱发转化为肿瘤相关纤维母细胞,发生遗传稳定性改变。炎性微环境及其骨髓间充质干细胞炎性信号通路异常在骨髓间充质干细胞的遗传稳定性改变中发挥重要作用,基于中医学温阳固肾、益气升阳、扶正祛邪理论,发现温阳益气类中药可明显提高肿瘤微环境及化学癌环境中骨髓间充质干细胞的遗传稳定性。
BACKGROUND：How to further definite and prevent the genetic instability of bone marrow mesenchymal stem cel s in tumor microenvironment has increasingly become a key problem for its clinic application. Meanwhile, increasing studies focus on the reciprocal causation relationship between uncontrol ed inflammation and tumors. OBJECTIVE：To analyze the changes of inflammation-related signaling pathways and genetic stability in bone marrow mesenchymal stem cel s under the tumor microenvironment, and meanwhile, to investigate the influence and mechanism of“warming yang and benefiting qi”method on inflammatory signaling pathways and the genetic stability of bone marrow mesenchymal stem cel s in tumor microenvironment, based on the guidance of the traditional Chinese Medicine theories“warming yang and tonifying kidney, benefiting qi and raising yang, strengthening healthy and discarding evil to eliminate pathogens”. METHODS：A computer-based online search of PubMed, CNKI and VIP was performed for relevant articles with the keywords of“bone marrow mesenchymal stem cel s, inflammatory balance, replenishing qi, warming yang, strengthening shen, elevate yang, tumor, cancer, yang can transform qi, yin shaping up body, genetic stability”in English and Chinese, respectively. Screened literatures were evaluated strictly according to the inclusion and exclusion criteria, and final y 42 articles were included in result analysis. RESULTS AND CONCLUSION：Under physical and chemical induction or tumor microenvironment, bone marrow mesenchymal stem cel s can promote tumor proliferation, invasion and metastasis. In addition, bone marrow mesenchymal stem cel s can be induced to differentiate into malignant cel s or tumor associating fibroblasts, fol owed by genetic instability, in tumor microenvironment. Inflammatory microenvironment plays an important role in regulating inflammation-related signals and genetic stability of bone marrow mesenchymal stem cel s. Chinese traditional medicines functioning as“warming yang and replenishing qi”can restore the genetic stability of bone marrow mesenchymal stem cel s in tumor or chemical microenvironment, based on the guidance of the traditional Chinese Medicine theories of“warming yang and tonifying kidney, benefiting qi and raising yang, strengthening healthy and discarding evil to eliminate pathogens”.