文献类型：期刊文献

英文题名：Cryopreservation of Dormant Buds by PVS-Based Vitrification: A Highly Promising Strategy for Large-Scale Cryobanking of Plant Genetic Resources

作者：Zhang, Yanhong H.[1];Wang, Jinxiu X.[1];He, Chunyu Y.[1];Guo, Qingyi Y.[1]

第一作者：张延红

通信作者：He, CY[1]

机构：[1]Gansu Univ Chinese Med, 35 Dingxi East Rd, Lanzhou 730000, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), Gansu Univ Chinese Med, 35 Dingxi East Rd, Lanzhou 730000, Peoples R China.|[10735]甘肃中医药大学;

年份：2025

外文期刊名：BIOPRESERVATION AND BIOBANKING

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001593601100001)】；

基金：This research was funded by the National Natural Science Foundation of China (No. 81960683), the Science andTechnology Project of Gansu Province (No. 21JR1RA263,24YANA007, 23YFFA0027, 24CXKA009, 22CX8NA082,and 25JRRA1178), and the Industrial Support Project of Colleges and Universities in Gansu Province (No. 2020C-09and 2024CYZC-40).

语种：英文

外文关键词：cryopreservation; dormant bud; physiological status; protocol optimization; PVS incubation; vitrification

摘要：Plant germplasm is critical for maintaining biodiversity and mitigating genetic erosion. While cryopreservation has emerged as an increasingly vital approach for conserving plant genetic resources, its routine application remains limited due to species- and explant-specific preservation requirements. In many instances, additional experimentation is necessary to develop genotype-specific protocols to achieve satisfactory survival rates. In addition, the technical operation is time-consuming and labor-intensive. Moreover, the survival rate of explants post-cryopreservation from many species remains suboptimal. PVS-based vitrification cryopreservation of dormant buds represents an ideal strategy to address these challenges, leveraging the buds' inherent cold hardiness and robust regrowth capacity. This review focuses on four key determinants of successful PVS-based cryopreservation: the collection time and physiological status of dormant buds, shoot tip dissection, PVS exposure duration, and recovery culture. The insights provided herein may facilitate the rapid development of new protocols or user-friendly procedures for a broader range of plant species, enabling wider adoption by institutions and laboratories, and thereby enhancing global efforts in germplasm conservation.