文献类型：期刊文献

中文题名：当归贝母苦参丸对顺铂化疗H22荷瘤小鼠肿瘤及肝脏、肾脏和胸腺组织病理形态的影响

英文题名：Effect of Danggui Beimu Kushen Pill on Pathological Morphology of Tumor, Liver, Kidney and Thymus of H22 Tumor-bearing Mice Treated with Cisplatin

作者：舍雅莉[1];闫德祺[1];刘永琦[1,2];李应东[1];李豆[1];程小丽[1]

第一作者：舍雅莉

机构：[1]甘肃中医学院系统生物学与中医药转化研究所,甘肃兰州730000;[2]甘肃中医学院基础医学院,甘肃省高校重大疾病分子医学与中医药防治研究省级重点实验室,甘肃兰州730000

第一机构：甘肃中医药大学科研实验中心（甘肃省中医药标准化技术委员会秘书处）

年份：2014

卷号：21

期号：5

起止页码：56

中文期刊名：中国中医药信息杂志

外文期刊名：Chinese Journal of Information on Traditional Chinese Medicine

收录：CSTPCD;;CSCD:【CSCD_E2013_2014】；

基金：国家科技支撑计划(2011BAI05B02);甘肃省高校基本科研业务费基金(甘财2009-192-3)

语种：中文

中文关键词：当归贝母苦参丸;H22荷瘤小鼠;肝脏;肾脏;胸腺

外文关键词：Danggui Beimu Kushen pill;H22 tumor-beaing mice;liver;kidney;thymus

摘要：目的观察当归贝母苦参丸对顺铂化疗H22荷瘤小鼠肿瘤及肝脏、肾脏和胸腺组织病理形态的影响,探讨其对顺铂化疗的增效减毒作用。方法建立小鼠H22肝癌皮下移植瘤模型,随机分为模型组、顺铂组、当归贝母苦参丸组、当归贝母苦参丸联合顺铂组,另设空白组作对照。造模后第8日开始给药,连续14 d,动态监测荷瘤小鼠肿瘤体积;第15日处死小鼠,镜下观察各组小鼠肿瘤、肝脏、肾脏和胸腺组织的病理形态变化。结果给药第10日,与模型组比较,各给药组肿瘤体积明显减小(P<0.01),当归贝母苦参丸联合顺铂组最为明显;各给药组肿瘤细胞密度、异型性和病理性核分裂像均明显降低(P<0.01),其中当归贝母苦参丸联合顺铂组效果优于顺铂组,还伴有明显的淋巴细胞浸润和纤维组织增生(P<0.01)。顺铂组肝脏、肾脏和胸腺病理变化较空白组、模型组加重(P<0.01),而当归贝母苦参丸联合顺铂组病变较顺铂组减轻(P<0.05)。结论当归贝母苦参丸可以增强顺铂对H22荷瘤小鼠肿瘤的抑制作用,减轻顺铂化疗H22荷瘤小鼠的肝脏、肾脏和胸腺毒性。
Objective To investigate the effect of Danggui Beimu Kushen pill （angelica, fritillaria, sophorae） on the pathological morphology of tumor, liver, kidney and thymus of cisplatin-treated H22 tumor-bearing mice, and explore its effect-enhancing and toxic-reducing action for cisplatin. Methods H22 tumor-bearing mice models were established, and randomized into model group, cisplatin group, Danggui Beimu Kushen pill group, Danggui Beimu Kushen pill combined cisplatin group, with normal group as control. Drugs were administrated from the eighth day for 14 d, the tumor volume of tumor-bearing mice was dynamically monitored. On the 15th days, the mice were killed to observe the pathological changes of tumor, liver, kidney and thymus under the microscope. Results After treatment for 10 d, compared with model group, the tumor volume of each treatment group was significantly decreased （P〈0.01）, Danggui Beimu Kushen pill group was most obvious. Compared with model group, the tumor cellularities, atypia and irregular mitosis of each treatment group were significantly decreased （P〈0.01）, the effect of Danggui Beimu Kushen pill combined cisplatin group was better than that of cisplatin group, accompanied by obvious lymphocyte infiltration and hyperplasia of fibrous tissue （P〈0.01）. The pathomorphological damages of liver, kidney and thymus in cisplatin group were obviously aggravating than normal group and model group （P〈0.01）, however the degree of damages in Danggui Beimu Kushen pill combined cisplatin group were reduced （P〈0.05）. Conclusion Danggui Beimu Kushen pill can not only enhance the antitumor effect of cisplatin, but also reduce cisplatin toxicity to liver, kidney and thymus of H22 tumor-bearing mice.