文献类型：期刊文献

英文题名：Circular RNA hsa_circ_0004396 acts as a sponge of miR-615-5p to promote non-small cell lung cancer progression and radioresistance through the upregulation of P21-Activated Kinase 1

作者：Li, Dong[1];Yan, Lin[2];Zhang, Junhan[3];Gu, Feng[4]

第一作者：Li, Dong

通信作者：Gu, F[1]

机构：[1]Gansu Prov Tumor Hosp, Dept Thorac Surg, Lanzhou, Gansu, Peoples R China;[2]Gansu Prov Hosp, Dept Anesthesiol, Lanzhou, Gansu, Peoples R China;[3]Gansu Univ Chinese Med, Res & Expt Ctr, Lanzhou, Gansu, Peoples R China;[4]Gansu Prov Tumor Hosp, Dept Aspirat Oncol, 2 East Xiaoxihu St, Lanzhou 730050, Gansu, Peoples R China

第一机构：Gansu Prov Tumor Hosp, Dept Thorac Surg, Lanzhou, Gansu, Peoples R China

通信机构：[1]corresponding author), Gansu Prov Tumor Hosp, Dept Aspirat Oncol, 2 East Xiaoxihu St, Lanzhou 730050, Gansu, Peoples R China.

年份：2022

卷号：36

期号：6

外文期刊名：JOURNAL OF CLINICAL LABORATORY ANALYSIS

收录：;Scopus(收录号：2-s2.0-85129176509);WOS:【SCI-EXPANDED(收录号:WOS:000789603900001)】；

语种：英文

外文关键词：miR-615-5p; PAK1; circRNA hsa_circ_0004396; non-small cell lung cancer

摘要：Backgrounds CircRNA hsa_circ_0004396 has been confirmed to be upregulated in human non-small cell lung cancer (NSCLC). The aim of his study was to evaluate its mechanism in the radioresistance and progression of NSCLC. Methods Hsa_circ_0004396, miR-615-5p, and P21-Activated Kinase 1 (PAK1) were measured by reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR). The binding between miR-615-5p and hsa_circ_0004396 or PAK1 was predicted by circinteractome or Targetscan, as verified by dual-luciferase reporter assay and RIP assay. Proliferation, clonogenicity capacity, cell cycle progression, apoptosis, migration, and invasion were assessed by CCK-8, colony formation, flow cytometry, and Transwell assay. Bcl-2, Bcl-2 associated protein X (Bax), MMP-2, and PAK1 protein levels were detected using western blot assay. In addition, in vivo function of hsa_circ_0004396 was evaluated by tumor xenograft assay. Results Hsa_circ_0004396 and PAK1 levels were upregulated, while miR-615-5p was declined in NSCLC. Hsa_circ_0004396 silencing inhibited NSCLC cell malignant behavior and induced radiosensitivity. Hsa_circ_0004396 functions as a molecular sponge of miR-615-5p to regulate PAK1 expression. Moreover, hsa_circ_0004396 knockdown inhibited NSCLC tumor growth in vivo. Conclusion Our findings demonstrated that hsa_circ_0004396 promoted NSCLC development and radioresistance through the miR-615-5p/PAK1 axis, which might provide a new therapeutic target for NSCLC treatment.