文献类型：期刊文献

英文题名：Neuropeptide S Attenuates the Alarm Pheromone-Evoked Defensive and Risk Assessment Behaviors Through Activation of Cognate Receptor-Expressing Neurons in the Posterior Medial Amygdala

作者：Shao, Yu-Feng[1,2];Wang, Can[1,8];Rao, Xiao-Ping[3];Wang, Hua-Dong[4,5];Ren, Yan-Li[1];Li, Jing[1,9];Dong, Chao-Yu[1,10];Xie, Jun-Fan[1];Yang, Xing-Wen[1];Xu, Fu-Qiang[3,4,5,6,7];Hou, Yi-Ping[1,2]

第一作者：Shao, Yu-Feng

通信作者：Hou, YP[1];Hou, YP[2]

机构：[1]Lanzhou Univ, Dept Neurosci Anat Histol & Embryol, Key Lab Preclin Study New Drugs Gansu Prov, Sch Basic Med Sci, Lanzhou, Peoples R China;[2]Lanzhou Univ, Key Lab Neurol Gansu Prov, Lanzhou, Peoples R China;[3]Chinese Acad Sci, Ctr Brain Sci, Natl Ctr Magnet Resonance Wuhan,State Key Lab Mag, Key Lab Magnet Resonance Biol Syst,Innovat Acad P, Wuhan, Peoples R China;[4]Chinese Acad Sci, Shenzhen Inst Adv Technol,Shenzhen Hong Kong Inst, Shenzhen Key Lab Neuropsychiat Modulat,Brain Cogn, CAS Key Lab Brain Connectome & Manipulat,Guangdon, Shenzhen, Peoples R China;[5]Chinese Acad Sci, Shenzhen Inst Adv Technol,Shenzhen Hong Kong Inst, Collaborat Innovat Ctr Brain Sci,Brain Cognit & B, CAS Key Lab Brain Connectome & Manipulat,Guangdon, Shenzhen, Peoples R China;[6]Huazhong Univ Sci & Technol, Wuhan Natl Lab Optoelect, Wuhan, Peoples R China;[7]Chinese Acad Sci, Ctr Excellence Brain Sci & Intelligence Technol, Shanghai, Peoples R China;[8]Weifang Med Univ, Dept Anat, Weifang, Peoples R China;[9]Gansu Univ Chinese Med, Dept Anat Histol & Embrylolgy, Sch Basic Med Sci, Lanzhou, Peoples R China;[10]Third Peoples Hosp Yunnan Prov, Dept Gynaecol, Kunming, Yunnan, Peoples R China

第一机构：Lanzhou Univ, Dept Neurosci Anat Histol & Embryol, Key Lab Preclin Study New Drugs Gansu Prov, Sch Basic Med Sci, Lanzhou, Peoples R China

通信机构：[1]corresponding author), Lanzhou Univ, Dept Neurosci Anat Histol & Embryol, Key Lab Preclin Study New Drugs Gansu Prov, Sch Basic Med Sci, Lanzhou, Peoples R China;[2]corresponding author), Lanzhou Univ, Key Lab Neurol Gansu Prov, Lanzhou, Peoples R China.

年份：2021

卷号：14

外文期刊名：FRONTIERS IN MOLECULAR NEUROSCIENCE

收录：;Scopus(收录号：2-s2.0-85122473016);WOS:【SCI-EXPANDED(收录号:WOS:000744039100001)】；

基金：This work was supported by the National Natural Science Foundation of China (Grant Nos. 31872770, 31830035, 82001396, 81771426, 31771198, 31500853, 81471347, 31400946, 81171254, and 81071076), the Key-Area Research and Development Program of Guangdong Province (Grant No. 2018B030331001), the Strategic Priority Research Program of Chinese Academy of Sciences (Grant No. XDB32030200), the Fundamental Research Funds for the Central University (Grant Nos. lzujbky-2019-cd03, lzujbky-2018-25, lzujbky-2017-135, and lzujbky-2015-277), and the Open-ended Fund of the Key Lab of Neurology of Gansu Province (Grant No. 21GKLN61602).

语种：英文

外文关键词：neuropeptide S; neuropeptide S receptor; alarm pheromone; neural circuit tracing; herpes simplex virus; posterior medial amygdala; antagonist; c-Fos

摘要：Neuropeptide S (NPS) acts by activating its cognate receptor (NPSR). High level expression of NPSR in the posterior medial amygdala suggests that NPS-NPSR system should be involved in regulation of social behaviors induced by social pheromones. The present study was undertaken to investigate the effects of central administration of NPS or with NPSR antagonist on the alarm pheromone (AP)-evoked defensive and risk assessment behaviors in mice. Furthermore, H129-H8, a novel high-brightness anterograde multiple trans-synaptic virus, c-Fos and NPSR immunostaining were employed to reveal the involved neurocircuits and targets of NPS action. The mice exposed to AP displayed an enhancement in defensive and risk assessment behaviors. NPS (0.1-1 nmol) intracerebroventricular (i.c.v.) injection significantly attenuated the AP-evoked defensive and risk assessment behaviors. NPSR antagonist [D-Val(5)]NPS at the dose of 40 nmol completely blocked the effect of 0.5 nmol of NPS which showed the best effective among dose range. The H129-H8-labeled neurons were observed in the bilateral posterodorsal medial amygdala (MePD) and posteroventral medial amygdala (MePV) 72 h after the virus injection into the unilateral olfactory bulb (OB), suggesting that the MePD and MePV receive olfactory information inputs from the OB. The percentage of H129-H8-labeled neurons that also express NPSR were 90.27 +/- 3.56% and 91.67 +/- 2.46% in the MePD and MePV, respectively. NPS (0.5 nmol, i.c.v.) remarkably increased the number of Fos immunoreactive (-ir) neurons in the MePD and MePV, and the majority of NPS-induced Fos-ir neurons also expressed NPSR. The behavior characteristic of NPS or with [D-Val(5)]NPS can be better replicated in MePD/MePV local injection within lower dose. The present findings demonstrated that NPS, via selective activation of the neurons bearing NPSR in the posterior medial amygdala, attenuates the AP-evoked defensive and risk assessment behaviors in mice.