文献类型：期刊文献

中文题名：Pingwei capsules improve gastrointestinal motility in rats with functional dyspepsia

英文题名：Pingwei capsules improve gastrointestinal motility in rats with functional dyspepsia

作者：Liang Qiankun[1];Mao Lanfang[2];Du Xiaojuan[2];Li Yunxia[1];Yan Yuan[1];Liang Jingjing[1];Liu Junhong[2];Wang Longde[2];Li Hongfang[1,3]

第一作者：Liang Qiankun

通信作者：Li, HF[1];Li, HF[2]

机构：[1]Lanzhou Univ, Coll Basic Med, Dept Physiol, Lanzhou 730000, Gansu, Peoples R China;[2]Gansu Chinese Med Coll, Affiliated Hosp, Dept Gastroenterol, Lanzhou 730020, Gansu, Peoples R China;[3]Key Lab Preclin Study New Drugs Gansu Prov, Lanzhou 730000, Gansu, Peoples R China

第一机构：Lanzhou Univ, Coll Basic Med, Dept Physiol, Lanzhou 730000, Gansu, Peoples R China

通信机构：[1]corresponding author), Lanzhou Univ, Coll Basic Med, Dept Physiol, Lanzhou 730000, Gansu, Peoples R China;[2]corresponding author), Key Lab Preclin Study New Drugs Gansu Prov, Lanzhou 730000, Gansu, Peoples R China.

年份：2018

卷号：38

期号：1

起止页码：43

中文期刊名：Journal of Traditional Chinese Medicine

外文期刊名：JOURNAL OF TRADITIONAL CHINESE MEDICINE

收录：;Scopus(收录号：2-s2.0-85042845786);WOS:【SCI-EXPANDED(收录号:WOS:000425480900005)】；CSCD:【CSCD2017_2018】；PubMed;

基金：Supported by the Natural Science Foundation of China (Based on Brain-Gut Axis to Study Peptidomics of Liver Stagnation and Spleen Deficiency with Functional Dyspepsia and Its Intervention of Shugan Jianpi Method, No. 81360540), And the Administration of Gansu Traditional Chinese Medicine and Chinese Herbs (A Study about Intervening Effects of a Chinese Herbal Preparation with Resveratrol on Insulin Resistance of Type 2 Diabetes Mellitus, No. GZK-2015-23)

语种：英文

中文关键词：消化不良;活动性;胃肠;老鼠;leptin;聚合酶链反应;十二指肠;CCK

外文关键词：Pingwei capsule; Dyspepsia; Gastrointestinal motility; Brain-gut peptide; Brain-gut axis

摘要：OBJECTIVE: To investigate the mechanism of Ping-wei capsules(PWC) in improving gastrointestinal m otility in rats with functional dyspepsia(FD).METHODS: We established an FD model by stimu-lating semi-starvation rats via tail damping, provo-cation, and forced exercise fatigue. The FD model group was further divided into five groups accord-ing to the treatment received: normal saline, dom-peridone, low-dose PWC, mid-dose PWC, or highdose PWC. The effect of PWC on FD was evaluated by measuring gastrointestinal motility. Changes in leptin and cholecystokinin(CCK) were detected through enzyme-linked immunosorbent assay, re-verse transcription-polymerase chain reaction, and immunohistochemistry.RESULTS: PWC significantly increased gastrointesti-nal m otility in FD rats. Furthermore, PWC signifi-cantly increased CCK m RNA and protein concentra-tions in the duodenum and antrum, decreased leptin protein concentrations in the duodenum, an-trum, and hypothalamus, and decreased CCK pro-tein concentration in the hypothalamus.CONCLUSION: PWC improves gastrointestinal mo-tor function in FD rats by decreasing the leptin con-centration in serum and the brain-gut axis, and by increasing the CCK concentration in gastrointesti-nal tissue. Our findings help to elucidate the mech-anism of FD and provide further insight into the pharmacokinetics of PWC.
OBJECTIVE: To investigate the mechanism of Pingwei capsules (PWC) in improving gastrointestinal motility in rats with functional dyspepsia (FD). METHODS: We established an FD model by stimulating semi-starvation rats via tail damping, provocation, and forced exercise fatigue. The FD model group was further divided into five groups according to the treatment received: normal saline, dom-peridone, low-dose PWC, mid-dose PWC, or high-dose PWC. The effect of PWC on FD was evaluated by measuring gastrointestinal motility. Changes in leptin and cholecystokinin (CCK) were detected through enzyme-linked immunosorbent assay, reverse transcription-polymerase chain reaction, and immunohistochemistry. RESULTS: PWC significantly increased gastrointestinal motility in FD rats. Furthermore, PWC significantly increased CCK mRNA and protein concentrations in the duodenum and antrum, decreased leptin protein concentrations in the duodenum, antrum, and hypothalamus, and decreased CCK protein concentration in the hypothalamus. CONCLUSION: PWC improves gastrointestinal motor function in FD rats by decreasing the leptin concentration in serum and the brain-gut axis, and by increasing the CCK concentration in gastrointestinal tissue. Our findings help to elucidate the mechanism of FD and provide further insight into the pharmacokinetics of PWC. (C) 2018 JTCM. All rights reserved.