文献类型：期刊文献

中文题名：小苞黄芪多糖的分离、结构表征及抗氧化活性研究

英文题名：Study on the isolation,structural characterization and antioxidant activity of polysaccharides from Astragalus prattii Simps

作者：王莉[1];游亚[2];王蕊霞[2];段国建[2]

第一作者：王莉

机构：[1]贵阳学院理学院,贵州贵阳550005;[2]甘肃中医药大学药学院,甘肃兰州730101

第一机构：贵阳学院理学院,贵州贵阳550005

年份：2025

卷号：42

期号：2

起止页码：36

中文期刊名：甘肃中医药大学学报

外文期刊名：Journal of Gansu University of Chinese Medicine

基金：2024年甘肃省高校大学生就业创业提升工程项目(202402);甘肃省科技计划项目(24JRRA883);甘肃省教育厅产业支撑计划项目(2025CYZC-050)。

语种：中文

中文关键词：小苞黄芪;多糖;结构表征;抗氧化活性;自由基清除;Fe2+螯合

外文关键词：Astragalus pratti Simps;polysaccharides;structural characterization;antioxidant activity;free radical scavenging;Fe^(2+)chelation

摘要：目的研究小苞黄芪多糖的抗氧化活性。方法采用水提分级醇沉法对小苞黄芪多糖进行提取和纯化,利用多种分析方法对20%乙醇浓度小苞黄芪多糖(APS20)、40%乙醇浓度小苞黄芪多糖(APS40)、60%乙醇浓度小苞黄芪多糖(APS60)、80%乙醇浓度小苞黄芪多糖(APS80)进行结构表征,并测定小苞黄芪多糖对1,1-二苯基-2-三硝基苯肼(DPPH)自由基、2,2’-联氮双(3-乙基苯并噻唑啉-6-磺酸)二铵盐(ABTS)自由基、羟基自由基的清除能力,以及对Fe^(2+)的螯合能力。结果测得4种小苞黄芪多糖的糖含量依次为63.2%,68.7%,73.4%,65.9%,且均包括甘露糖(Man)、鼠李糖(Rha)、葡萄糖醛酸(GlcA)、半乳糖醛酸(GalA)、葡萄糖(Glu)、半乳糖(Gal)和阿拉伯糖(Ara)等7种单糖,分子量主要分布在3.0×10^(3)~3.2×10^(4)Da之间,表观均为片状;红外光谱结果显示,4种小苞黄芪多糖都含有一定量的糖醛酸,并具有α、β2种构型,核磁共振结果进一步说明APS60以β型糖苷键为主。抗氧化活性研究结果显示,4种小苞黄芪多糖对多种自由基均有较好的清除能力,尤其对Fe^(2+)螯合能力明显优于L-抗坏血酸(VC)。结论小苞黄芪多糖能够有效减少自由基的产生,保护细胞免受氧化损伤,从而对人体的健康产生积极的影响,为深入开发小苞黄芪多糖的药用价值提供试验依据。
Objective To study the antioxidant activity of Astragalus prattii Simps polysaccharide(APS).Methods The polysaccharides were extracted and purified through water extraction and graded alcohol precipitation.Four APS fractions(APS20,APS40,APS60,and APS80)obtained at ethanol concentrations of 20%,40%,60%,and 80%respectively underwent comprehensive structural characterization using multiple analytical techniques.The scavenging ability of 1,1-diphenyl-2-trinitrophenylhydrazine(DPPH)free radicals,2,2'-diazide(3-ethylbenzothiazolin-6-sulfonic acid)diamiammonium salts(ABTS)free radicals,hydroxyl free radicals,and the chelating ability of Fe^(2+)were derermined.Results It demonstrated sugar contents of 63.2%,68.7%,73.4%,and 65.9%for the four APS fractions,all containing seven monosaccharide components:mannose(Man),rhamnose(Rha),glucuronic acid(GlcA),galacturonic acid(GalA),glucose(Glu),galactose(Gal),and arabinose(Ara).Molecular weight distributions ranged from 3.0×10^(3)to 3.2×10^(4)Da,with all samples exhibiting flaky morphology.Fourier-transform infrared spectroscopy(FT-IR)analysis confirmed the presence of uronic acids and bothα-andβ-configurations,while nuclear magnetic resonance(NMR)results specifically indicatedβ-glycosidic bond dominance in APS60.Antioxidant assays revealed superior radical scavenging capabilities across all APS fractions,particularly demonstrating significantly enhanced Fe^(2+)chelation efficiency compared to L-ascorbic acid(VC).Conslusion These findings substantiate that APS effectively reduces free radical generation and protects cells from oxidative damage,providing experimental evidence for further development of its medicinal value.