详细信息
大黄牡丹汤组方对急性胰腺炎模型大鼠腺泡细胞分泌功能的影响 被引量:13
The Effect of Dahuang Mudan Decoction on Exocrine Function of Pancreatic Acinar Cells in Acute Pancreatitis Model Rats
文献类型:期刊文献
中文题名:大黄牡丹汤组方对急性胰腺炎模型大鼠腺泡细胞分泌功能的影响
英文题名:The Effect of Dahuang Mudan Decoction on Exocrine Function of Pancreatic Acinar Cells in Acute Pancreatitis Model Rats
作者:张延英[1];舒畅[1];蔡兴[2,3];吴建军[1];张艳霞[1];王茸[1];李存祥[1];康万荣[1];王荣[1]
第一作者:张延英
机构:[1]甘肃中医学院/甘肃省中药药理与毒理学重点实验室/甘肃省高校中(藏)药化学与质量研究省级重点实验室,兰州730000;[2]甘肃省分析测试中心;[3]甘肃省实验动物管理委员会,兰州730000
第一机构:甘肃中医药大学科研实验中心(甘肃省中医药标准化技术委员会秘书处)
年份:2014
卷号:31
期号:3
起止页码:26
中文期刊名:实验动物科学
外文期刊名:Laboratory Animal Science
收录:CSTPCD
基金:甘肃省技术研究与开发专项(No.1305TCYA043)
语种:中文
中文关键词:大黄牡丹汤组方;急性胰腺炎;胰腺腺泡细胞;AP模型大鼠;FI超载
外文关键词:Dahuang Mudan Decoction; acute pancreatitis(AP) ; pancreatic acinar cell; AP model rats; overload of FI
摘要:目的研究大黄牡丹汤组方(RPDP)对急性胰腺炎(Acute pancreatitis,AP)模型大鼠胰腺腺泡细胞外分泌功能和腺泡细胞内钙离子浓度(FI)的影响。方法 SD大鼠灌胃RPDP以制备大黄牡丹汤组方含药血清(RPDP-S);SD大鼠分为假手术组和AP模型组,分离胰腺腺泡细胞并与RPDP-S共同孵育,测定腺泡细胞淀粉酶分泌水平和FI变化。结果 AP模型大鼠腺泡细胞分泌淀粉酶水平较假手术组显著升高(P<0.05),经RPDP-S处理的AP模型大鼠腺泡细胞分泌淀粉酶水平显著降低(P<0.05);FI随AP模型病程延长而升高(P<0.05),RPDP-S可抑制AP模型大鼠腺泡细胞内FI升高(P<0.05)。结论 RPDP通能抑制AP大鼠胰腺腺泡细胞的外分泌功能,抑制腺泡细胞内钙离子的升高,降低腺泡细胞内FI超载。
Objective To study the effect of Dahuang Mudan Decoction (RPDP) on exocrine function and intracellular Ca2+ concentration(FI) of pancreatic acinar cells in acute pancreatitis (AP) model rats.Method Dahuang Mudan Decoction was administrated to SD rats and the serum containing Dahuang Mudan Decoction (RPDP-S) wasprepared.SD rats randomly divided into three groups:the normal control group,the sham operation (SO) group and the AP group.Separate pancreatic acinar cells were isolated and co-incubated with RPDP-S,amylase secretion of pancreatic acinar cells and the change of FI were analyzed.Result Compared with the SO group,amylase secretion of pancreatic acinar cells was significantly increase in AP group(P < O.05) ; and amylase secretion was significantly decrease in the pancreatic acinar cells treated with RPDP-S(P < O.05),and the FI was raise up along with the prolonged disease (P < 0.05).Conclusion RPDP can decrease intracellular Ca2+ concentration,and inhibit the overload of FIand the exocrine function of pancreatic acinar cells.
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