文献类型：期刊文献

中文题名：麻花秦艽休眠芽离体培养时的丛生芽发生途径研究

英文题名：Study on Dormant Culture and Plant Regeneration via Cluster Buds of Gentiana straminea

作者：张延红[1];高素芳[1]

第一作者：张延红

机构：[1]甘肃中医药大学药学院

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

年份：2015

卷号：0

期号：9

起止页码：1804

中文期刊名：中药材

外文期刊名：Journal of Chinese Medicinal Materials

收录：MEDLINE(收录号：26930974);CSTPCD;;Scopus;北大核心:【北大核心2014】；CSCD:【CSCD2015_2016】；PubMed;

基金：甘肃省自然科学基金(BH2012-076);甘肃省中医药管理局项目(GZK-2010-Z2)

语种：中文

中文关键词：麻花秦艽;休眠芽;丛生芽;生根培养

外文关键词：Gentiana straminea Maxim.; Dormant bud; Cluster buds; Root induction

摘要：目的:以休眠芽为外植体,建立麻花秦艽丛生芽再生的实验技术体系,为麻花秦艽种苗工厂化离体快繁和种质资源离体保存提供理论依据和技术支撑。方法:以MS为基本培养基,添加不同浓度和种类的细胞分裂素和生长素,诱导休眠芽产生丛生芽。以1/2MS为基本培养基,添加不同浓度和种类的生长素诱导丛生芽生根。结果:MS+2.0 mg/L 6-BA+0.01 mg/L NAA+30 g/L蔗糖+7 g/L琼脂培养基是诱导休眠芽产生丛生芽及丛生芽继代的最佳培养基,丛生芽诱导率为93.3%,丛芽经继代培养后芽增殖系数为5.6。最佳生根培养基是1/2MS(无机盐减半)+2.0 mg/L IAA+0.5 mg/L IBA+15 g/L蔗糖+7 g/L琼脂,生根率达93.5%,再生植株生长健壮。结论:该试验建立了以休眠芽为外植体的麻花秦艽丛生芽再生植株的技术体系。
Objective： In order to provide scientific basis for micropropagation and cryopreservation of Gentiana straminea,plantlets were regenerated from dormant buds by cluster buds. Methods： Based on MS medium,dormant buds were inoculated in mediums containing different type and concentration of cytokinin and auxin for inducing cluster buds. 1 /2MS medium with different concentration of auxin were used for inducing root. Results： The medium of MS with 2. 0 mg/L 6-BA,0. 01 mg/L NAA,30 g/L sucrose and 7 g/L agar was suitable for cluster buds＇ primary culture and subculture. The cluster buds inducing rate was 93. 3%. Multiple shoot clumps multiplication factor was 5. 6. The medium of 1 /2MS with 2. 0 mg/L IAA,0. 5 mg/L IBA,15 g/L sucrose and 7 g/L agar was suitable for root induction,its inducing rate was 93. 5% with plantlets growing well. Conclusion： Plantlets regenerated from dormant buds of Gentiana straminea via cluster buds are established in this study.