文献类型：期刊文献

中文题名：藁本内酯衍生物对软骨细胞外基质合成与分解代谢影响

英文题名：Effects of ligusticum cycloprolactam on synthesis and catabolism of extracellular matrix in chondrocytes

作者：刘建军[1];陈欣[1,2];汪欣月[3];齐伟[2];王多贤[1];席芳琴[1];李宁[1,2];柳军玺[4];谢兴文[1,2]

第一作者：刘建军

机构：[1]甘肃中医药大学附属医院,甘肃兰州730000;[2]甘肃中医药大学,甘肃兰州730000;[3]北京中医药大学,北京100029;[4]中国科学院兰州化学物理研究所,甘肃兰州730000

第一机构：甘肃中医药大学第二附属医院

年份：2023

卷号：29

期号：11

起止页码：1615

中文期刊名：中国骨质疏松杂志

外文期刊名：Chinese Journal of Osteoporosis

收录：CSTPCD;;北大核心:【北大核心2020】；CSCD:【CSCD2023_2024】；

基金：甘肃省青年科技基金计划(20JR10RA345);兰州市城关区科技计划项目(2020-2-2-7);甘肃中医药大学附属医院院内创新基金(gzfy-2019-12);甘肃省科技计划资助(20JR5RA129);兰州市科技发展指导性计划项目(2020-ZD-65),甘肃省拔尖领军人才项目。

语种：中文

中文关键词：骨关节炎;藁本内酯衍生物;软骨细胞外基质;白细胞介素-1β

外文关键词：osteoarthritis;ligusticum cycloprolactam;Cartilage extracellular matrix;Interleukin-1β

摘要：目的探究藁本内酯衍生物(LIGc)对IL-1β诱导大鼠软骨细胞外基质合成与分解代谢的影响。方法将大鼠软骨细胞分为空白对照组、IL-1β组及LIGc高剂量组(0.4μmol/mL)、中剂量组(0.2μmol/mL)、低剂量组(0.1μmol/mL)组。CCK-8法检测细胞活力;细胞免疫荧光检测COL2α1和ACAN的表达;采用RT-qPCR检测软骨细胞中SOX9、PRG4、MMP-13、ADAMTS-5的基因表达水平。Western blot检测软骨细胞中HMGB1、COX2、TLR4、NF-κB p65的蛋白表达水平。结果与正常组比较,IL-1β组软骨细胞增殖降低;COL2α1和ACAN的表达降低;SOX9、PRG4 mRNA表达下调,同时MMP-13、ADAMTS-5 mRNA表达上调;HMGB1、COX2、TLR4、NF-κB p65蛋白表达升高。与IL-1β组比较,LIGc能够促进软骨细胞的增殖;增强COL2α1和ACAN的表达;上调SOX9、PRG4 mRNA表达,同时下调MMP-13、ADAMTS-5 mRNA表达;下调HMGB1、COX2、TLR4、NF-κB p65蛋白表达。结论LIGc能够抑制IL-1β所致炎症因子表达,上调软骨细胞外基质合成代谢因子、下调分解代谢因子延缓软骨细胞外基质降解的作用,其机制可能与OA免疫炎症反应的TLR4/NF-κB信号通路相关。
Objective To investigate the effect of ligusticum cycloprolactam(LIGc)on IL-1β-induced extracellular matrix synthesis and catabolism in rat cartilage.Methods Rat chondrocytes were divided into blank control group,IL-1βgroup and LIGc high-dose(0.4μmol/mL),medium-dose(0.2μmol/mL)and low-dose(0.1μmol/mL)groups;cell viability was detected by CCK-8 assay;cell immunofluorescence was performed to detect the expression of COL2α1 and ACAN;and chondrocyte gene expression levels were detected by RT-qPCR.gene expression levels of SOX9,PRG4,MMP-13 and ADAMTS-5 in the cells.The protein expression levels of HMGB1,COX2,TLR4,and NF-κB p65 in chondrocytes were detected by Western blot.Results Compared with the normal group,chondrocyte proliferation was reduced in the IL-1βgroup;the expression of COL2α1 and ACAN was decreased;the mRNA expression of SOX9 and PRG4 was downregulated,whereas the mRNA expression of MMP-13 and ADAMTS-5 was upregulated;and the protein expression of HMGB1,COX2,TLR4 and NF-κB p65 was increased.Compared with the IL-1βgroup,LIGc was able to promote the proliferation of chondrocytes;increase the expression of COL2α1 and ACAN.The expression of SOX9 and PRG4 mRNA was upregulated,while the expression of MMP-13 and ADAMTS-5 mRNA was downregulated.The protein expression of HMGB1,COX2,TLR4 and NF-κB p65 was downregulated.Conclusion LIGc can inhibit the expression of inflammatory factors induced by IL-1β,upregulate the anabolic factor of chondrocyte extracellular matrix and down-regulate the catabolic factor to delay the degradation of chondrocyte extracellular matrix.The mechanism may be related to the TLR4/NF-κB signalling pathway of the immune inflammatory response in osteoarthritis.