文献类型：期刊文献

英文题名：Study on complications of osteoporosis based on network pharmacology

作者：Song, Zhijing[1,2,3];Zhang, Haoling[4];Jiang, Yuhang[5];Zhao, Rui[1];Pei, Xuedong[1];Ning, Haochi[1];Chen, Hailiang[1];Pan, Jing[1];Gong, Yanlong[2];Song, Min[1];Wang, Wei[6]

第一作者：宋志靖;Song, Zhijing

通信作者：Song, M[1];Wang, W[2]

机构：[1]Gansu Univ Chinese Med, Clin Coll Chinese Med, Lanzhou, Gansu, Peoples R China;[2]Gansu Univ Chinese Med, Affiliated Hosp, Lanzhou, Gansu, Peoples R China;[3]Minist Educ, Key Lab Dunhuang Med, Lanzhou, Gansu, Peoples R China;[4]St Petersburg State Univ, St Petersburg, Russia;[5]Gansu Univ Chinese Med, Sch Publ Hlth, Lanzhou, Gansu, Peoples R China;[6]Gansu Univ Chinese Med, Coll Acupuncture Moxibust & Tuing, Lanzhou, Gansu, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), Gansu Univ Chinese Med, Clin Coll Chinese Med, Lanzhou, Gansu, Peoples R China;[2]corresponding author), Gansu Univ Chinese Med, Coll Acupuncture Moxibust & Tuing, Lanzhou, Gansu, Peoples R China.|[10735]甘肃中医药大学;

年份：2022

卷号：13

外文期刊名：FRONTIERS IN GENETICS

收录：;Scopus(收录号：2-s2.0-85139983815);WOS:【SCI-EXPANDED(收录号:WOS:000868662300001)】；

基金：1. National Natural Science Foundation of China: Response mechanism of MC3T3-E1 cell stress transduction signal and cytoskeleton and intervention effect of Guben Zenggu granules under continuous static pressure, No. 81960877. 2. Natural Science Foundation of Gansu Province: The effect of high altitude environment on osteogenesis of osteoporosis rats and the intervention effect of Guben Zenggu Granules. No. 21JR7RA561. 3. Innovation Fund of Universities in Gansu Province: Study on the mechanism of low mechanical stress affecting MC3T3-E1 cell activity through TRIM21/F-Actin signal and the intervention effect of Guben Zenggu Pill containing drug serum. No. 2021A-076. 4. Open Fund project of Dunhuang Key Laboratory of Medicine and Transformation of Ministry of Education, Research and Development of Dunhuang Mofeng ointment and in vitro pharmacodynamics evaluation, No. DHYX20-16.

语种：英文

外文关键词：network pharmacology; gene sequencing; guben zenggu granules; continuous static pressure; MC3T3-E1 cells

摘要：Osteoporosis is a serious threat to human life. Guben Zenggu Granule is an empirical prescription for clinical treatment of osteoporosis. MC3T3-E1 cells are mouse osteogenic precursor cells with osteogenic differentiation, and are classic cells for studying bone metabolism and osteogenic mechanism, as well as mechanical stimulation sensitive cells. Therefore, it can be inferred that Guben Zenggu granule can repair MC3T3-E1 cells under continuous static pressure overload. This study aims to through the network of pharmacology and gene sequencing method, reveal thrift increase bone particles under the condition of continuous static pressure overload on osteogenesis mechanism of MC3T3-E1 cells. In the process of analysis, from a variety of 98 compounds was predicted in the database, a collection of 474 goals, a total of 29,164 difference between two groups of genes. Then, construction of composite targets between cells and predict targets and protein - protein interaction networks, and through the cluster analysis to further explore the relationship between the target. In addition, linkages between target proteins and cells were further identified using Gene Ontology (GO) and Pathways (KEGG Pathway). Finally, the repair effect of Guben Zenggu granule on MC3T3-E1 cells under continuous static pressure overload was verified through experiments, so as to accurately explain the pharmacodynamic mechanism of Traditional Chinese medicine.