文献类型：期刊文献

中文题名：正交试验法优选通便胶囊的提取工艺条件

英文题名：Optimization of Extracting Process Conditions of Tongbian Capsule by Orthogonal Test

作者：宋治荣[1];王兰霞[2];李士博[2]

第一作者：宋治荣

机构：[1]甘肃中医学院,兰州730000;[2]甘肃药品检验所,兰州730000

第一机构：甘肃中医药大学

年份：2010

卷号：27

期号：2

起止页码：123

中文期刊名：中国现代应用药学

外文期刊名：Chinese Journal of Modern Applied Pharmacy

收录：CSTPCD;;北大核心:【北大核心2008】；CSCD:【CSCD_E2011_2012】；

语种：中文

中文关键词：通便胶囊;药效试验;正交试验;盐酸小檗碱;高效液相色谱法

外文关键词：Tongbian capsule; pharmacodynamic study; orthogonal test; berberine hydrochloride; HPLC

摘要：目的优选通便胶囊中木香、黄连、槟榔的提取工艺条件。方法结合中医理论,通过药效学试验验证,比较醇提物、水提物在小鼠肠推进方面的作用,确定提取工艺,并以干膏率、盐酸小檗碱的量为评价指标,采用L9(34)正交表对影响提取的因素醇浓度、用量、提取次数、时间进行考察。结果优选工艺条件为药材加10倍量30%乙醇,提取3次,每次1.5 h。结论经药效学实验验证、正交试验筛选得到的提取工艺条件科学合理可行。
OBJECTIVE To optimize the extraction process conditions from Costas,Coptis and Betel nut of Tongbian capsule.METHODS Combined of traditional Chinese medicine theory and passed pharmacodynamic study to compare ethanol extraction with water extraction to determine ethanolic route in the stimulative role of mice intestinal.Content of extraction and berberine hydrochloride are regarded as the assessing index to determine the optimum extracting conditions.Content of ethanol,amount of ethanol,extracting times and extracting time are selected by orthogonal design and the analysis of variance.RESULTS The optimum conditions of extraction process are 30% ethanol,which is 10 times of the amount of herb material,refluxed 3 times,1.5 h for each time.CONCLUSION The optimal extracting process conditions are scientific,reasonable and feasible by pharmacodynamic study and orthogonal test.