文献类型：期刊文献

中文题名：苦马豆素对胃癌细胞中pten和survivin mRNA表达的影响

英文题名：Influence of Swainsonine on the Expressions of Pten and Survivin mRNA in the Cells of Gastric Cancer

作者：王宏伟[1];程小丽[2];许雅清[2];邱家权[2];李海龙[2];吴玉泓[2];徐燕[2]

第一作者：王宏伟

机构：[1]甘肃中医学院附属医院检验科,甘肃兰州730020;[2]甘肃中医学院

第一机构：甘肃中医药大学第二附属医院

年份：2015

卷号：28

期号：12

起止页码：25

中文期刊名：西部中医药

外文期刊名：Western Journal of Traditional Chinese Medicine

收录：CSTPCD

基金：兰州市城关区科技发展计划项目(编号2011-1-10)

语种：中文

中文关键词：苦马豆素;胃癌;基因表达

外文关键词：swainsonine; gastric cancer; genctic expression

摘要：目的:探讨不同浓度的疯草提取物苦马豆素对胃癌SGC-7901、MKN-45细胞增殖的抑制作用以及对pten和survivin基因的影响。方法:以不同浓度的疯草提取物苦马豆素处理胃癌SGC-7901、MKN-45细胞后,采用MTT法测定细胞活力;以流式细胞术检测细胞凋亡;并用RT-q PCR法检测pten和survivin基因的表达。结果:不同浓度的疯草提取物苦马豆素均能抑制细胞增殖,1.00 mg/m L的苦马豆素可明显诱导胃癌SGC-7901、MKN-45细胞发生凋亡,其凋亡率分别达到了40.94%和43.21%;RT-q PCR法检测显示pten基因表达上调,survivin基因表达下调。结论:疯草提取物苦马豆素抑制胃癌细胞增殖并诱发凋亡,其原因可能与上调pten和下调survivin m RNA的表达有关。
Objective： To explore the influence of swainsonine in different concentrations on pten and survivin genes and inhibitory action on cellular proliferation of SGC-7901 and MKN-45 of gastric cancer. Methods： Cellular activity was detected by adopting MTT method after processing SGC-7901 and MKN-45 cells with swainsonine in different concentrations; cellular apoptosis was detected by flow cytometry; the expressions of pten and surviving were measured by RT- q PCR method. Results： S wainsonine in different concentrations could inhibit cellular proliferation, and swainsonine in the concentration of 1.00 mg/m L could obviously induce the apoptosis of SGC-7901 and MKN-45, apoptosis rates reached 40.94% and 43.21%; the results of RT-q PCR method revealed that the expressions of pten gene upregulated, the expressions of survivin gene down-regulated. Conclusion： Swainsonine could inhibit the proliferation of gastric cancer cells and induce its apoptosis, and the reason might be related to upregulating pten expressions and downregulating surviving m RNA expressions.