文献类型：期刊文献

中文题名：秦艽寒热不同配伍对风寒湿痹类风湿关节炎大鼠白细胞介素-6及血管内皮生长因子的影响

英文题名：Effects of Different Compatibility of Gentianae Macrophyllae Radix on IL-6 and Vascular Endothelial Growth Factor in Rheumatoid Arthritis Rats with Wind-cold-dampness Arthralgia

作者：苏杉[1];王蓉[1];赵敏[1];吴晨[1];高慧琴[1]

第一作者：苏杉

机构：[1]甘肃中医药大学

第一机构：甘肃中医药大学

年份：2019

卷号：26

期号：3

起止页码：39

中文期刊名：中国中医药信息杂志

外文期刊名：Chinese Journal of Information on Traditional Chinese Medicine

收录：CSTPCD;;CSCD:【CSCD_E2019_2020】；

基金：国家自然科学基金(81360648)

语种：中文

中文关键词：秦艽;风寒湿痹;类风湿关节炎;白细胞介素-6;血管内皮生长因子;大鼠

外文关键词：Gentianae Macrophyllae Radix;wind-cold-dampness arthralgia;rheumatoid arthritis;interleukin-6;vascular endothelial growth factorarthritis;rats

摘要：目的观察秦艽不同配伍药对对风寒湿痹型类风湿关节炎(RA)模型大鼠白细胞介素-6(IL-6)及血管内皮生长因子(VEGF)的影响,从中药药性及复方配伍理论的角度揭示中药药性-病证-功效的关联性。方法 80只SD大鼠随机分为空白对照组、Ⅱ型胶原组、病证模型组、阳性对照组、单味秦艽组、秦威组(秦艽配伍威灵仙)、秦桑组(秦艽配伍桑寄生)、秦防组(秦艽配伍防己),每组10只。注射Ⅱ型胶原乳剂并施加风寒湿刺激制备风寒湿型RA大鼠模型。造模完成后各给药组给予相应药液,空白对照组、Ⅱ型胶原组及病证模型组给予生理盐水。实验过程中,记录大鼠一般状况;每3 d测量1次大鼠左后足跖厚度,并计算足跖肿胀度;实验第38日大鼠进行关节炎指数(AI)评分;ELISA检测大鼠血清IL-6含量;Westernblot和RT-PCR分别检测大鼠踝关节VEGF的蛋白和m RNA表达。结果与空白组比较,Ⅱ型胶原组和病证模型组足跖肿胀度、AI评分、血清IL-6含量、踝关节VEGF蛋白和m RNA表达明显增加(P<0.01);与病证模型组比较,各中药组足跖肿胀度、AI评分、血清IL-6含量、踝关节VEGF蛋白和m RNA表达均有所减少,其中秦威组最明显(P<0.01)。结论对于风寒湿痹型RA,药性平温相配疗效优于平平相配、平寒相配及单味秦艽,实验结果与中医"疗寒以热药"的原则基本相符。
Objective To observe the effects of different compatibility of Gentianae Macrophyllae Radix (Qinjiao) on IL-6 and vascular endothelial growth factor (VEGF) in rheumatoid arthritis (RA) model rats with wind-cold-dampness arthralgia. To reveal the correlation between TCM property-syndrome-effect from the perspective of TCM properties and compound compatibility theory. Methods Totally 80 healthy SD rats were randomly divided into blank control group, collage Ⅱ model group, wind-cold-dampness syndrome model group, positive control group, single-taste Qinjiao group, Qinjiao-Weilingxian group (Qinwei group), Qinjiao-Sangjisheng group (Qinsang group), Qinjiao-Fangji Group (Qinfang group), 10 rats in each group. Rat model of wind-cold-dampness type RA was established by injecting type Ⅱ collagen emulsion and applying wind-cold-dampness stimulus. After the establishment of the model, the blank control group, collage Ⅱ model group and wind-cold-dampness syndrome model group were given normal saline, and the corresponding liquid medicine was given to each administration group. In the experiment, the general state of rats was recorded. The thickness of the left posterior metatarsal of rats was measured every 3 days, and the swelling degree of the metatarsal was calculated. The arthritis index (AI) was evaluated on the 38th day of the experiment. The content of IL-6 in serum of rats was detected by ELISA. Expression of VEGF in ankle joint of rats was detected by Western blot. Expression of VEGF mRNA in ankle joint was detected by RT-PCR. Results Compared with the blank group, the swelling degree, AI score, serum IL-6 content, VEGF protein expression level and VEGF mRNA expression in collage Ⅱ model group and wind-cold-dampness syndrome model group were significantly increased ( P <0.01). Compared with the wind-cold-dampness syndrome model group, the foot swelling degree, AI score, serum IL-6 content, ankle VEGF protein expression level and VEGF mRNA expression of administration group were decreased, and Qinwei group was the most significant ( P <0.01). Conclusion For RA of wind-cold-dampness arthralgia type, the treatment efficacy of mild and warm is better than the combined effect of mild and cold TCM or mild TCM drugs. The experimental results are basically consistent with the principle of "treating cold diseases should use hot medicine" in TCM.