文献类型：期刊文献

英文题名：Lipid droplet-targeted fluorescent probes for polarity sensing in Ferroptosis cellular models and in vivo zebrafish

作者：Han, Zehua[1,2];Han, Taihe[1,2];Huang, Qin[1,2];Ma, Junbao[1,2];Mu, Shuai[1,2,3];Wang, Huayu[1,2];Liu, Xiaoyan[1,2];Wang, Xin[4];Zhang, Haixia[1,2]

第一作者：Han, Zehua

通信作者：Zhang, HX[1];Zhang, HX[2];Wang, X[3]

机构：[1]Lanzhou Univ, State Key Lab Appl Organ Chem, Lanzhou 730000, Peoples R China;[2]Lanzhou Univ, Coll Chem & Chem Engn, Lanzhou 730000, Peoples R China;[3]Lanzhou Univ, First Hosp Lanzhou Univ, Clin Med Coll 1, Med Frontier Innovat Res Ctr, Lanzhou 730000, Peoples R China;[4]Gansu Univ Chinese Med, Affiliated Hosp, Dept Urol, Lanzhou 730000, Peoples R China

第一机构：Lanzhou Univ, State Key Lab Appl Organ Chem, Lanzhou 730000, Peoples R China

通信机构：[1]corresponding author), Lanzhou Univ, State Key Lab Appl Organ Chem, Lanzhou 730000, Peoples R China;[2]corresponding author), Lanzhou Univ, Coll Chem & Chem Engn, Lanzhou 730000, Peoples R China;[3]corresponding author), Gansu Univ Chinese Med, Affiliated Hosp, Dept Urol, Lanzhou 730000, Peoples R China.|[10735b845793de6ae2b30]甘肃中医药大学第二附属医院;[10735]甘肃中医药大学;

年份：2026

卷号：348

外文期刊名：SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001611739000001)】；

基金：This work was supported by the National Natural Science Foundation of China (NSFC) Fund (no. 22574069) and The Major Science and Technology Project of Gansu Province Fund (no. 23ZDNA007) .

语种：英文

外文关键词：Fluorescent probes; Lipid droplet polarity; Ferroptosis; Zebrafish model

摘要：Changes in the polarity of the lipid droplets (LDs) microenvironment are closely associated with pathological processes such as ferroptosis. However, existing probes suffer from limitations like small Stokes shifts, narrow polarity response ranges, and insufficient targeting specificity, making dynamic and accurate monitoring challenging. To address this, we developed three fluorescent probes (LD-H1, LD-H2, LD-H3) through rational molecular design. Among them, LD-H3 exhibits outstanding performance with a large Stokes shift (60 nm redshift), ideal lipophilicity (logP = 6.61), and exceptional targeting specificity (Pearson coefficient > 0.97), enabling precise monitoring of LDs polarity dynamics. Cellular imaging demonstrated that LD-H3 can clearly capture the dynamic changes of increasing LDs polarity (4.7-fold) and shrinking morphology during ferroptosis, and it shows high sensitivity responses to different polarity stimuli (serum-free conditions, DMSO, oleic acid). In vivo experiments using zebrafish further validated the specific labeling capability of LD-H3 with high contrast and low background interference. This study provides LD-H3 as a superior probe for deciphering LDs polarity dynamics in complex pathophysiological environments, offering valuable insights into ferroptosis mechanisms.