文献类型：期刊文献

中文题名：秦巴硒菇提取物硒蛋白多糖荷瘤药物血清促慢性红白血病细胞株K562凋亡的实验研究

英文题名：Study on apoptosis of K562 cells induced by tumor-bearing herb serum of selenium-protein polysaccharide extracted from Qinba selenium-mushroom(秦巴硒菇)

作者：楚惠媛[1];陈彻[2]

第一作者：楚惠媛

机构：[1]甘肃中医学院生命医学实验室;[2]甘肃中医学院科研中心

第一机构：甘肃中医药大学

年份：2006

卷号：13

期号：3

起止页码：142

中文期刊名：中国中西医结合急救杂志

外文期刊名：Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care

收录：CSTPCD

基金：甘肃省教育厅自然科学基金资助项目(02404)

语种：中文

中文关键词：秦巴硒菇;硒蛋白多糖;细胞凋亡,caspase-3

外文关键词：Qinba selenium-mushroom; selenium-protein polysaccharide; apoptosis; caspase-3

摘要：目的:探讨秦巴硒菇提取物硒蛋白多糖制备的含药血清对慢性红白血病细胞株(K 562)凋亡的影响。方法:建立荷瘤小鼠模型,采用血清药理学方法制备硒蛋白多糖含药血清,常规培养K 562细胞。通过甲基噻唑基四唑(M TT)比色法检测硒蛋白多糖含药血清对肿瘤生长的抑制率;用形态学和DNA电泳技术观察细胞凋亡的发生;用比色法检测凋亡相关基因caspase 3的表达。结果:秦巴硒菇提取物硒蛋白多糖含药血清可显著抑制K 562细胞生长,且呈时间剂量依赖性。形态学、DNA电泳结果均证实有凋亡发生,与空白血清组比较,caspase 3基因上调明显。结论:秦巴硒菇提取物硒蛋白多糖含药血清可促进K 562细胞凋亡,其机制可能与上调caspase 3基因有关。
Objective： To explore the effect of selenium-protein polysaccharide extracted from Qinba selenium-mushroom （秦巴硒菇） on apoptosis of K562 cells. Methods： Tumor-bearing mice model was established, and selenium protein polysaccharide extracted from Qinba selenium-mushroom （contained-herb serum） was prepared by the method of serum pharmacology. K562 cells were conventionally cultured. Growth inhibition rate of tumor cells was detected by methyl thiazolyl tetrazolium （MTT） method. Morphological changes of apoptotic cell were observed under a fluorescent microscope. Cell apoptosis was observed by DNA electrophoresis. Caspase - 3 relative gene was measured by colorimetry. Results： Selenium-protein polysaccharide extracted from Qinba selenium mushroom could significantly inhibit the growth of K562 cells, there was a remarkably positive correlation between drug concentration, time and inhibitory rate. Apoptotic phenomenon was certained via morphological examination and DNA electrophoresis. Compared with control group, caspase-3 gene was markedly upregulated. Conclusion： Selenium-protein polysaccharide extracted from Qinba selenium-mushroom could induce apoptosis of K562 cells. The mechanism may be related to upregulation of caspase-3.