文献类型：期刊文献

中文题名：非奈利酮通过Toll样受体4/髓样分化因子88/核因子κB信号通路抑制高糖诱导人肾小球足细胞炎症损伤的研究

英文题名：Study on the inhibitory effect of Finerenone on high glucose induced inflammatory injury of human glomerular podocytes through the Toll-like receptor 4/myeloid differentiation factor 88/nuclear factorκB signaling pathway

作者：刘胜男[1,2];王紫琼[1];何文静[1];王丽娟[1];马红梅[1];武振亚[1];汤惠惠[1];杨文[1];王金羊[3]

第一作者：刘胜男

机构：[1]甘肃中医药大学第一临床医学院,兰州7300O0;[2]甘肃武威肿瘤医院兰州院区放疗三科;[3]甘肃省人民医院内分泌科干部病区

第一机构：甘肃中医药大学临床医学院

年份：2026

卷号：34

期号：3

起止页码：217

中文期刊名：中国糖尿病杂志

外文期刊名：Chinese Journal of Diabetes

收录：;北大核心:【北大核心2023】；

基金：国家自然科学基金(81760147、81560143、82360163);甘肃省自然科学基金(24JRRA589);甘肃省卫生健康行业项目(GSWSQN2024?01)。

语种：中文

中文关键词：非奈利酮;高糖;肾小球足细胞;Toll样受体4/髓样分化因子88/核因子κB

外文关键词：Finerenone;High glucose;Glomerular podocytes;Toll like receptor 4/myeloid differentiation factor 88/nuclear factorκB

摘要：目的探讨非奈利酮通过Toll样受体4/髓样分化因子88/核因子κB(TLR4/My D88/NF?κB)信号通路,改善高糖(HG)诱导人肾小球足细胞(HGPC)损伤的机制。方法体外培养HGPC分为正常对照(NC)组、HG组、非奈利酮(Fin)组。免疫荧光检测TLR4、My D88、NF?κB、盐皮质激素受体(MR)及Nephrin蛋白表达,q RT?PCR检测TLR4、My D88、NF?κB及IL?1βmRNA表达,ELISA检测TNF?α水平。结果HG组TLR4、My D88、NF?κB及MR蛋白荧光表达高于NC、Fin组(P<0.05),Nephrin蛋白荧光表达低于NC、Fin组(P<0.05)。HG组TLR4、My D88、NF?κB、IL?1βmRNA表达及TNF?α高于NC、Fin组(P<0.05)。结论非奈利酮通过下调TLR4/My D88/NF?κB信号通路,减轻HG诱导的HGPC炎症损伤。
Objective To investigate the mechanism of Finerenone in improving high glucose(HG)induced human glomerular podocyte(HGPC)injury through Toll-like receptor 4/myeloid differentiation factor 88/nuclear factorκB(TLR4/MyD88/NF-κB)signaling pathway.Methods HGPC cultured in vitro were divided into normal control(NC)group,HG group and Finerenone(Fin)group.Immunofluorescence was used to detect the protein expression of TLR4,MyD88,NF-κB,mineralocorticoid receptor(MR)and Nephrin.The mRNA expressions of TLR4,MyD88,NF-kB and IL-1βwere detected by qRT-PCR,and the level of tumor necrosis factorα(TNF-α)was detected by ELISA.Results The protein fluorescence expression of TLR4,MyD88,NF-κB,and MR were significantly higher,while Nephrin was significantly lower in the HG group than in the NC and Fin groups(P<0.05).The mRNA expression of TLR4,MyD88,NF-κB,IL-1βand TNF-αwere higher in HGgroup than in NC and Fin groups(P<0.05).Conclusions Finerenone alleviates HG-induced inflammatory injury of HGPC by down regulating TLR4/MyD88/NF-κB signaling pathway.