文献类型：期刊文献

中文题名：干旱胁迫下外源硅对蒙古黄芪幼苗生理生化特性的影响

英文题名：Effects of Silicon on Physiological and Biochemical Characteristics of Astragalus membranaceus Seedlings under Drought Stress

作者：王惠珍[1];陆国弟[1];胡茹英[1];孙强[1];陈红刚[1];王引权[1]

第一作者：王惠珍

机构：[1]甘肃中医药大学,甘肃兰州730000

第一机构：甘肃中医药大学

年份：2020

卷号：27

期号：3

起止页码：67

中文期刊名：中国中医药信息杂志

外文期刊名：Chinese Journal of Information on Traditional Chinese Medicine

收录：CSTPCD;;CSCD:【CSCD_E2019_2020】；

基金：西北中藏药协同中心项目(XBXT2015-4);甘肃省青年科技基金计划(17JR5RA166);现代农业技术体系专项资金(CARS-21)。

语种：中文

中文关键词：蒙古黄芪;干旱胁迫;硅;渗透调节物质;丙二醛;抗氧化酶

外文关键词：Astragalus membranaceus;drought stress;silicon;osmotic regulators;malondialdehyde;antioxidant enzymes

摘要：目的研究不同处理条件下蒙古黄芪幼苗的生理特性变化,探讨干旱胁迫下外源硅对蒙古黄芪幼苗影响的生理机制。方法采用沙培试验,设置3个聚乙二醇模拟浓度和3个硅浓度,测定不同条件下蒙古黄芪幼苗叶绿素、丙二醛、渗透调节物质、抗氧化酶指标,研究干旱胁迫下外源硅对蒙古黄芪幼苗生理生化特性的影响。结果随干旱胁迫增加,叶绿素总量和叶绿素a呈先增加后降低趋势,叶绿素b呈降低趋势;加入外源硅使叶绿素含量呈显著增加趋势。随干旱胁迫增加,丙二醛含量呈增加趋势,重度、中度胁迫分别较对照增加53.7%、40.6%;加入外源硅可减少丙二醛含量。随干旱胁迫增加,游离脯氨酸(Pro)呈增加趋势,中度胁迫为对照的1.7倍,重度胁迫分别为对照、中度胁迫的3.5、2.1倍;加入外源硅可使重度胁迫下Pro含量显著升高。随干旱胁迫增加,超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性整体呈增加趋势;加入外源硅使SOD活性降低但不显著,POD在中度胁迫下显著降低,CAT在重度胁迫下显著降低。且以硅浓度为6 mmol/L时作用最明显。结论在干旱胁迫下,外源硅可使Pro处于更高水平,降低丙二醛含量,提高抗氧化酶活性,促进叶绿素合成,有利于蒙古黄芪幼苗生长。
Objective To study changes of physiological characteristics of Astragalus membranaceus seedlings under drought stress;To explore the physiological mechanism of silicon on seedlings of Astragalus membranaceus under drought stress.Methods Sand culture test was adopted.The effects of silicon on physiological and biochemical characteristics of Astragalus membranaceus seedlings under drought stress were studied by measuring chlorophyll,MDA,osmotic regulators and antioxidant enzymes in three PEG-6000 simulated concentrations and three silicon concentrations.Results With the increase of drought stress,the total contents of chlorophyll and chlorophyll a of seedlings increased at first and then decreased,and chlorophyll b decreased,and the chlorophyll content increased significantly with the addition of silicon.MDA increased by 53.7%and 40.6%under severe and moderate stress compared with CK,respectively;Silicon could reduce MDA production.Proline showed an increasing trend with the increase of drought stress.Moderate stress was 1.7 times as much as CK,and severe stress was 3.5 times and 2.1 times as much as CK and moderate stress;Silicon addition could significantly increase Proline content under severe stress.The activities of three antioxidant enzymes(SOD,POD and CAT)in seedlings increased as a whole with the increase of drought stress.With the addition of silicon,the activities of SOD decreased.POD decreased significantly under moderate stress,and CAT decreased significantly under severe stress.The most significant effect appeared when the concentration of silicon was 6 mmol/L.Conclusion Under drought stress,the application of silicon can make Proline at a higher level,reduce the content of MDA,increase the activity of antioxidant enzymes,and promote chlorophyll synthesis,which is beneficial to the growth of Astragalus membranaceus seedlings under drought stress.