文献类型：期刊文献

英文题名：Preparative Separation and Purification of Rebaudioside A from Steviol Glycosides Using Mixed-Mode Macroporous Adsorption Resins

作者：Lui, Yongfeng[1,2,3];Di, Duolong[1,2];Bai, Qingqing[4];Li, Jintian[4];Chen, Zhenbin[1,2,5];Lou, Song[1,2,3];Ye, Helin[1,2,3]

第一作者：Lui, Yongfeng

通信作者：Di, DL[1]

机构：[1]Chinese Acad Sci, Key Lab Chem NW Plant Resources, Lanzhou 730000, Peoples R China;[2]Chinese Acad Sci, Key Lab Nat Med Gansu Prov, Lanzhou Inst Chem Phys, Lanzhou 730000, Peoples R China;[3]Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China;[4]Gansu Coll Tradit Chinese Med, Dept Pharm, Lanzhou 730000, Peoples R China;[5]Lanzhou Univ Technol, Sch Mat Sci & Engn, Lanzhou 730050, Peoples R China

第一机构：Chinese Acad Sci, Key Lab Chem NW Plant Resources, Lanzhou 730000, Peoples R China

通信机构：[1]corresponding author), Chinese Acad Sci, Key Lab Chem NW Plant Resources, 18 Tianshui Middle Rd, Lanzhou 730000, Peoples R China.

年份：2011

卷号：59

期号：17

起止页码：9629

外文期刊名：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

收录：;EI(收录号：20113714326910);Scopus(收录号：2-s2.0-80052606184);WOS:【SCI-EXPANDED(收录号:WOS:000294585100072)】；

基金：This research project was financially supported by the National Natural Sciences Foundation of China (NSFC No. 20974116) and the "Hundred Talents Program" of the Chinese Academy of Sciences (CAS).

语种：英文

外文关键词：Rebaudioside A; mixed-mode macroporous adsorption resin; steviol glycoside; adsorption kinetics; adsorption isotherms; purification

摘要：Preparative separation and purification of rebaudioside A from steviol glycosides using mixed-mode macroporous adsorption resins (MARs) were systematically investigated. Mixed-mode MARs were prepared by a physical blending method. By evaluation of the adsorption/desorption ratio and adsorption/desorption capacity of mixed-mode MARs with different proportions toward RA and ST, the mixed-mode MAR 18 was chosen as the optimum strategy. On the basis of the static tests, it was found that the experimental data fitted best to the pseudosecond-order kinetics and Temkin-Pyzhev isotherm. Furthermore, the dynamic adsorption/desorption experiments were performed on the mini column Packed with mixed-mode MAR 18. After one run treatment, the purity of rebaudioside A in purified product increased from 40:77; to 60.53%, With a yield rate of 38.73% (W/W), and that in residual product decreased from 40.77 to 36.17%, with a recovery yield of 57.61% (W/W). The total recovery yield reached 9634% (W/W). The results showed that this method could be utilized in large-scale production of rebaudioside A from steviol glycosides in industry.