文献类型：期刊文献

英文题名：Peptide-based probe for colorimetric and fluorescent detection of Cu2+and S2-in environmental and biological systems

作者：An, Yong[1];Li, Linyu[1];Li, Lepeng[1];Sun, Yongqiang[1];Li, Bo[1,2];Wang, Peng[3]

第一作者：An, Yong

通信作者：Li, B[1];Wang, P[2]

机构：[1]Gansu Univ Chinese Med, Sch Clin Med 1, Lanzhou 730000, Gansu, Peoples R China;[2]Gansu Prov Hosp, Dept Musculoskeletal Tumor, Lanzhou 730000, Gansu, Peoples R China;[3]China West Normal Univ, Coll Chem & Chem Engn, Chem Synth & Pollut Control Key Lab Sichuan Prov, Nanchong 637009, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), Gansu Univ Chinese Med, Sch Clin Med 1, Lanzhou 730000, Gansu, Peoples R China;[2]corresponding author), China West Normal Univ, Coll Chem & Chem Engn, Chem Synth & Pollut Control Key Lab Sichuan Prov, Nanchong 637009, Peoples R China.|[10735]甘肃中医药大学;

年份：2024

卷号：465

外文期刊名：JOURNAL OF HAZARDOUS MATERIALS

收录：;EI(收录号：20235015200228);Scopus(收录号：2-s2.0-85179494650);WOS:【SCI-EXPANDED(收录号:WOS:001132939000001)】；

基金：This work were supported by the National Natural Science Foundation of China (NO. 82303568 and 22206154), Gansu Provincial Natural Science Foundation (NO.23JRRA1216), the Introduced Talent Research Start-up Fund (NO. 2023-YJRC-16) and Scientific Research and Innovation Fund (NO. 2021-KCYB-07) of Gansu University of Chinese Medicine.

语种：英文

外文关键词：Copper ions; Hydrogen sulfide; Colorimetry; On-site visualization; Peptide-based

摘要：Pollution caused by Copper and hydrogen sulfide pollution has severe adverse effects on the environment and organisms. Real-time, fast and accurate monitoring of Cu2+ and S2-faces serious challenges. In this study, we designed a novel biosensor and synthesized it by mimicking the structure of the main Cu(II)-binding site on bovine serum albumin. As a peptide-based sensor, FGGH (FITC-Gly-Gly-His-NH2) can perform the sequential detection of Cu2+ and S2- by fluorescence and colorimetry. The high water solubility and selectivity make it suitable for monitoring Cu2+ and S2- in environmental water samples with high sensitivity; its limit of detection (LOD) is as low as 1.42 nM for Cu2+ and 22.2 nM for S2-. The paper-based sensing platform of this probe was found to be a promising tool for the on-site visualization of real-time quantitative analysis of Cu2+ and S2- due to its rapid response and recyclable detection characteristics. Additionally, FGGH was successfully used to image Cu2+ and S2- in living cells and zebrafish models with adequate fluorescence stability and low cytotoxicity, providing the first visual evidence of the effect of the interactions between Cu2+ and S2- on the redox homeostasis of organisms.