文献类型：期刊文献

英文题名：Adenosine improves postmenopausal obesity by regulating neutrophil extracellular traps

作者：Wang, Anzhu[1];Zhang, Wei[2];Kang, Mengjiao[3];Wang, Shan[1];Shi, Jingjing[4];Xie, Pengfei[5];Zhao, Linhua[5,6];Tong, Xiaolin[6]

第一作者：Wang, Anzhu

通信作者：Zhao, LH[1];Zhao, LH[2];Tong, XL[2]

机构：[1]China Japan Friendship Hosp, Natl Ctr Integrat Med, Beijing 100029, Peoples R China;[2]Gansu Univ Chinese Med, Sch Basic Med, Lanzhou 730101, Gansu, Peoples R China;[3]Zhejiang Chinese Med Univ, Hangzhou 310053, Zhejiang, Peoples R China;[4]Guangzhou Univ Chinese Med, Sch Clin Med 2, Guangzhou 510405, Peoples R China;[5]China Acad Chinese Med Sci, Guanganmen Hosp, Inst Metab Dis, 5 Beixiange St, Beijing 100053, Peoples R China;[6]Changchun Univ Chinese Med, Affiliated Hosp, Dept Endocrinol, Changchun 130021, Jilin, Peoples R China

第一机构：China Japan Friendship Hosp, Natl Ctr Integrat Med, Beijing 100029, Peoples R China

通信机构：[1]corresponding author), China Acad Chinese Med Sci, Guanganmen Hosp, Inst Metab Dis, 5 Beixiange St, Beijing 100053, Peoples R China;[2]corresponding author), Changchun Univ Chinese Med, Affiliated Hosp, Dept Endocrinol, Changchun 130021, Jilin, Peoples R China.

年份：2025

卷号：15

期号：1

外文期刊名：SCIENTIFIC REPORTS

收录：;Scopus(收录号：2-s2.0-105010274998);WOS:【SCI-EXPANDED(收录号:WOS:001526529600015)】；

基金：This study was supported by grant from the Scientific and technological innovation project of China Academy of Chinese Medical Sciences (CI2021B008). This study was supported by grant from the Innovation Team and Talents Cultivation Program of National Administration of Traditional Chinese Medicine (No: ZYYCX-TD-D-202001).

语种：英文

外文关键词：Postmenopausal obesity; Adenosine; Neutrophil extracellular traps; Inflammatory response

摘要：Postmenopause marks a critical physiological transition in women, triggering changes in fat distribution and promoting obesity, which is strongly linked to systemic low-grade chronic inflammation, with neutrophil extracellular traps (NETs) playing a pivotal role in this process. Differentially expressed genes (DEGs) related to obesity were identified from the GEO database (GSE44000), followed by protein-protein interaction (PPI) network construction and functional enrichment analysis. Immune infiltration analysis was performed to assess changes in the adipose tissue microenvironment. In vivo, a bilateral ovariectomized (OVX) mouse model was employed to evaluate the effects of adenosine on obesity. RT-qPCR and Western blot were used to assess gene expression and NETs markers. A total of 510 DEGs were identified, with 469 upregulated genes primarily linked to inflammation and immune infiltration. PPI analysis highlighted key genes involved in NETs formation. Immune infiltration analysis revealed significant immune microenvironment alterations. Adenosine treatment in OVX mice reduced body weight, fat tissue, liver lipid deposition, and NETs markers (ELANE, MPO), downregulating TNF, ITGB2, and ITGAM. Adenosine ameliorates postmenopausal obesity by regulating markers of NETs, underscoring the critical role of inflammation in obesity pathogenesis and offering a potential novel therapeutic approach.