文献类型：期刊文献

英文题名：PLD3: In vitro functional characterization and autophagy pathway-mediated regulatory effects in gastric cancer

作者：Li, Yu[1];Geng, Jinrui[1];Liu, Na[1];Zhang, Yan[1];Dong, Xiao[1];Li, Yonghong[1,2]

第一作者：李阳;李颖;李洋;李杨;李芸;李燕;李勇;李晔;李瑶

通信作者：Li, YH[1]

机构：[1]Gansu Univ Chinese Med, Clin Med Coll 1, Lanzhou 730000, Peoples R China;[2]NHC Key Lab Diag & Therapy Gastrointestinal Tumor, Lanzhou 730000, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), Gansu Univ Chinese Med, Clin Med Coll 1, Lanzhou 730000, Peoples R China.|[10735]甘肃中医药大学;

年份：2026

卷号：811

外文期刊名：BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

收录：;Scopus(收录号：2-s2.0-105031574912);WOS:【SCI-EXPANDED(收录号:WOS:001709705200001)】；

基金：This work was supported by the National Natural Science Foundation of China (Grant No. 82160534) , Central Government Guided Fund for Local Science and Technology Development (Grant No. 25ZYJA003) , and Gansu Province Major Science and Technology Innovation Project in the Health Sector (Grant No. GSWSZD2024-01) , The Nature Science Foundation of Gansu Province (23JRRA1491) ,the Gansu Provincial Health Industry Project (Grant No. GSMSQN2021-004) .

语种：英文

外文关键词：Gastric cancer; Bioinformatics; PLD3; Vps34; Prognostic biomarker

摘要：Background: PLD3, a type-II transmembrane glycoprotein enriched in late endosomes and lysosomes, governs nucleic-acid turnover, immune signalling and intracellular homeostasis, and consequently influences both cell-cycle progression and programmed death. Although carcinoma of the stomach ranks among the most frequently diagnosed and lethal malignancies of the gastrointestinal tract, the precise functional dialogue between PLD3 and gastric tumorigenesis has yet to be elucidated. Methods: Bioinformatics workflows were applied to analyze gastric carcinoma datasets retrieved from The Cancer Genome Atlas (TCGA). Comprehensive analysis of PLD3 encompassed quantification of expression, survival outcomes, and analysis of its correlation with clinical stage and TNM staging system. RT-qPCR was employed to validate the expression levels of PLD3 in gastric carcinoma tissue and established cell lines. In vitro functional experiments were conducted to further investigate the biological functions of PLD3 in gastric cancer and its mechanistic association with autophagy. Results: Research shows PLD3 is markedly overexpressed in gastric carcinoma tissues, and its high expression is closely linked to reduced patient overall survival. Functional assays verified PLD3 knockdown notably impairs cancer cell malignant phenotypes; conversely, PLD3 overexpression boosts cancer cell malignancy and suppresses apoptosis. Treating PLD3-overexpressing gastric cancer cells with Vps34-IN-1-a potent inhibitor of autophagy key regulator Vps34-significantly inhibits PLD3-driven proliferation and promotes apoptosis. Conclusions: PLD3 exhibits markedly elevated expression in gastric cancer tissues, thus serving as a highly promising novel biomarker for prognostic assessment of this malignancy.