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HPLC同时测定雪松松针中没食子酸、原儿茶酸和儿茶素的含量     被引量:7

Simultaneous Determination of Gallic Acid, Protocatechuic Acid and Catechin in Pine Needles of Cedrus Deodara by HPLC

文献类型:期刊文献

中文题名:HPLC同时测定雪松松针中没食子酸、原儿茶酸和儿茶素的含量

英文题名:Simultaneous Determination of Gallic Acid, Protocatechuic Acid and Catechin in Pine Needles of Cedrus Deodara by HPLC

作者:邓毅[1];宁红霞[1];沈薇[2];石晓峰[1,2];李师[1];雷艳萍[1]

第一作者:邓毅

机构:[1]甘肃中医学院药学院,兰州730030;[2]甘肃省医学科学研究院药物研究所,兰州730050

第一机构:甘肃中医药大学药学院(西北中藏药协同创新中心办公室)

年份:2014

卷号:31

期号:9

起止页码:1104

中文期刊名:中国现代应用药学

外文期刊名:Chinese Journal of Modern Applied Pharmacy

收录:CSTPCD;;CSCD:【CSCD_E2013_2014】;

基金:甘肃省科技支撑计划项目(1204FKCA152);甘肃省高校中(藏)药化学与质量研究省级重点实验室开放基金项目(zzy-2011-05);甘肃省卫生厅中医药科研项目(GZK-2012-29)

语种:中文

中文关键词:雪松松针;没食子酸;原儿茶酸;儿茶素;含量测定;高效液相色谱法

外文关键词:pine needles of Cedrus deodara; gallic acid; protocatechuic acid; catechin; contents determination; HPLC

摘要:目的建立同时测定雪松松针中没食子酸、原儿茶酸和儿茶素含量的高效液相色谱法。方法采用TC-C18(250 mm×4.6 mm,5μm)色谱柱,流动相为甲醇(A)-0.2%磷酸水溶液(B)梯度洗脱(0~8 min,10%A,8~45 min,10%→30%A),流速:1.0 mL·min-1,检测波长:280 nm,柱温:25℃。结果雪松松针中没食子酸、原儿茶酸和儿茶素依次在0.4~4.0,4~40和2~20μg·mL-1内呈良好线性关系,相关系数r分别为0.999 8,0.999 9,0.999 9,加样回收率(n=9)分别为104.7%,99.5%和105.8%。结论该方法适用于雪松松针中没食子酸、原儿茶酸和儿茶素的含量测定,方法简单、快速、高效。
OBJECTIVE To develop an HPLC method for simultaneous determination of gallic acid, protocatechuic acid and catechin components in pine needles of Cedrus deodara. METHODS Agilent TC-C18 column (250 mm×4.6 mm,5μm) was adopted. The mobile phase was methanol (A)-0.2% phosphoric acid (B) with gradient elution (0-8 min, 10%A; 8-45 min, 10%→30%A) at the flow rate of 1.0 mL.min-1. The column temperature was 25 ℃ and the detection wavelength was 280 nm. RESULTS Calibration curves were found to be linear in the ranges of 0.4-4.0 μg.mL-1 for gallic acid, 4-40 μg.mL-1 for protocatechuic acid and 2-20 μg.mL-1 for catechin. The correlation coefficients were 0.999 8, 0.999 9, 0.999 9. The average recoveries(n=9) of gallic acid, protocatechuic acid and catechin were 104.7%, 99.5% and 105.8%. CONCLUSION The method is simple, rapid and efficient, which is suitable for the determination of gallic acid, protocatechuic acid and catechin components in pine needles of Cedrus deodara.

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