文献类型：期刊文献

英文题名：5'tiRNA-35-GlyTCC-3 and 5'tiRNA-33-CysGCA-11 target BMP6, CUL1 and SPR of non-syndromic cleft palate

作者：Liu, Ruimin[1,2];Zhang, Linxiang[1,2];Hu, Peinan[1,2];Liu, Anni[1,2];Zhang, Yixin[3];Liu, Qian[3];Guo, Jianqing[4];Han, Dong[5];Yue, Haiquan[2];Zhang, Baoping[1]

第一作者：Liu, Ruimin

通信作者：Zhang, BP[1];Yue, HQ[2];Han, D[3]

机构：[1]Lanzhou Univ, Sch Stomatol, Donggang West Rd 199, Lanzhou 730000, Peoples R China;[2]Gansu Prov Hosp, Dept Oral & Maxillofacial Surg, Lanzhou 730000, Peoples R China;[3]Gansu Univ Chinese Med, Clin Med Coll 1, Lanzhou 730000, Peoples R China;[4]Zhonghe 404 Hosp, Dept Stomatol, Jiayuguan 735100, Peoples R China;[5]Gansu Wuwei Tumor Hosp, Gansu Prov Wuwei Acad Med Sci, Wuwei 733000, Peoples R China

第一机构：Lanzhou Univ, Sch Stomatol, Donggang West Rd 199, Lanzhou 730000, Peoples R China

通信机构：[1]corresponding author), Lanzhou Univ, Sch Stomatol, Donggang West Rd 199, Lanzhou 730000, Peoples R China;[2]corresponding author), Gansu Prov Hosp, Dept Oral & Maxillofacial Surg, Lanzhou 730000, Peoples R China;[3]corresponding author), Gansu Wuwei Tumor Hosp, Gansu Prov Wuwei Acad Med Sci, Wuwei 733000, Peoples R China.

年份：2025

卷号：25

期号：1

外文期刊名：BMC ORAL HEALTH

收录：;Scopus(收录号：2-s2.0-85218903838);WOS:【SCI-EXPANDED(收录号:WOS:001434992400004)】；

基金：The work was supported by the National Natural Science Foundation of Gansu Province (23JRRA1080); Natural Science Foundation of Gansu Province (23JRRA1289); Internal Fund of Gansu Provincial People's Hospital (ZX-62000001-2022-259); Fundamental Research Funds for the scientific research fund of School (Hospital) of Stomatology Lanzhou University ((22)0660; lzukqky-2022-t12; lzukqky-2022-t16); Internal Fund of Gansu Provincial People's Hospital (22GSSYD-44).

语种：英文

外文关键词：tsRNA; Non-syndromic cleft palate; Microarray analysis; Enrichment analysis; RT-qPCR

摘要：BackgroundtsRNAs are novel small non-coding RNAs that play important regulatory roles in gene expression, translation, transcription, and epigenetic modification through proteins or mRNAs and may be therapeutic targets for certain diseases. The etiology of non-syndromic cleft palate-only is complex and the pathogenesis is poorly understood, non-coding RNAs play important roles in its development.MethodsThe tsRNAs of patients with simple cleft palate were compared with healthy individuals using small RNA microarray, bioinformatic analysis, quantitative real-time transcription polymerase chain reaction, and the effects measured using immunohistochemical staining.ResultsSeventy-nine tsRNAs were upregulated and fifty-four tsRNAs were downregulated in patients with simple cleft palate compared with healthy individuals, among which the expression of 5'tiRNA-35-GlyTCC-3 and 5'tiRNA-33-CysGCA-11 was markedly different and was involved in key signaling pathways related to the development of the palate, such as the cell cycle, cAMP signaling pathway, BMP signal transduction, folate biosynthesis, and other key signaling pathways that determine anatomical structure occurrence, regulate gene expression during development, influence epigenetics, and other biological processes, its target genes include BMP6, CUL1 and SPR.Conclusion5'tiRNA-35-GlyTCC-3 and 5'tiRNA-33-CysGCA-11 are closely associated with non-syndromic cleft palate development and are expected to be potential new targets for diagnosis and treatment.