文献类型：期刊文献

中文题名：氧化苦参碱对慢性乙型病毒性肝炎Toll样受体9信号通路的影响

英文题名：Effects of Oxymatrine in vitro on Toll-like Receptor 9 Signal Transduction Pathway in PBMCs from Chronic Hepatitis B Patients

作者：姚凝[1]

第一作者：姚凝

机构：[1]甘肃中医学院临床医学院,甘肃兰州730000

第一机构：甘肃中医药大学临床医学院

年份：2014

卷号：21

期号：7

起止页码：28

中文期刊名：中国中医药信息杂志

外文期刊名：Chinese Journal of Information on Traditional Chinese Medicine

收录：CSTPCD;;CSCD:【CSCD_E2013_2014】；

基金：甘肃省自然科学基金(0710RJZA010)

语种：中文

中文关键词：乙型肝炎病毒;Toll样受体信号通路;氧化苦参碱;细胞因子

外文关键词：hepatitis B virus;toll-like receptor signal pathway;oxymatrine;cytokine

摘要：目的探讨氧化苦参碱抗乙型肝炎病毒(HBV)感染的作用机制。方法分离提取慢性乙型病毒性肝炎患者外周血单个核细胞(PBMCs),在体外加入氧化苦参碱刺激,检测其诱导抗病毒细胞因子产生的能力,及对Toll样受体9(TLR9)信号通路表达及功能的调节作用。结果氧化苦参碱可直接刺激PBMCs分泌大量干扰素α(IFN-α)和干扰素γ(IFN-γ),上调PBMCs细胞TLR9信号通路中TLR9、髓样分化因子88及肿瘤坏死因子受体相关因子6等信号传递分子,并诱导信号通路活化释放大量抗病毒细胞因子(IFN-α、IFN-γ和肿瘤坏死因子-α)。结论氧化苦参碱通过直接诱导抗病毒细胞因子释放及上调活化TLR9信号通路发挥其抗HBV作用。
Objective To investigate the anti-HBV mechanism of oxymatrine in vitro.Methods The antiviral mechanism of oxymatrine was analyzed through detecting the expression and function of TLR9 signal pathway and the secretion of antiviral cytokines from PBMCs treated by oxymatrine in vitro.Results Oxymatrine could not only induce IFN-α and IFN-γ secretions from PBMCs directly, but also effectively augmented the expressions of TLR9, MyD88 and TRAF6 at mRNA level, and activated the TLR9 signal transduction pathway. TLR9 ligand could induce IFN-α, IFN-γ and TNF-α secretion a lot from PBMCs pretreated by oxymatrine.Conclusion Oxymatrine possesses an anti-HBV activity via activation of TLR9 signal pathway and secretion of antiviral cytokines.