文献类型：期刊文献

英文题名：Effect of Total Flavonoids of Oxytropis falcata Bunge on the Expression of p-JAK1-and p-STAT1-Related Proteins in Idiopathic Pulmonary Fibrosis

作者：Wang, Yan-jun[1,2,3];Li, Yang[1];Wang, Xue-lin[1,2,3];Li, Xin-ze[1,2,3];Chen, Yan-wen[1];Yang, Ling-ling[1];Ming, Hai-xia[1,2,3,4]

第一作者：Wang, Yan-jun;王玉娟;王亚军;王亚娟;王勇军

通信作者：Ming, HX[1];Ming, HX[2];Ming, HX[3];Ming, HX[4]

机构：[1]Gansu Univ Chinese Med, Lanzhou 730000, Peoples R China;[2]Prov Level Key Lab Mol Med Major Dis & Prevent &, Lanzhou 730000, Peoples R China;[3]Lab Chinese Med Pharmacol & Toxicol, Lanzhou 730000, Peoples R China;[4]Gansu Univ Tradit Chinese Med, Lanzhou 730000, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), Gansu Univ Chinese Med, Lanzhou 730000, Peoples R China;[2]corresponding author), Prov Level Key Lab Mol Med Major Dis & Prevent &, Lanzhou 730000, Peoples R China;[3]corresponding author), Lab Chinese Med Pharmacol & Toxicol, Lanzhou 730000, Peoples R China;[4]corresponding author), Gansu Univ Tradit Chinese Med, Lanzhou 730000, Peoples R China.|[10735]甘肃中医药大学;

年份：2020

卷号：2020

外文期刊名：EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000570958100001)】；

基金：This study was funded by the National Natural Science Foundation of China (no. 81860830).

语种：英文

摘要：Objective. The study aimed to explore the effect of total flavonoids ofOxytropis falcataBunge (FOFB) on the expression of p-JAK1/p-STAT1 and SOCS3 proteins in idiopathic pulmonary fibrosis (IPF).Methods. Rats model with IPF was established by one-off intratracheal injection of bleomycin (BLM, 5 mg/kg). After 14 days, the same volume of low dose (100 mg/kg), medium dose (200 mg/kg), and high dose (400 mg/kg) of FOFB and prednisolone acetate (20 mg/kg) as positive control drugs, as well as normal saline, were orally administered to rats once a day for 28 consecutive days. Subsequently, the degree of fibrosis and alveolitis in rat lung tissue was observed, respectively, by HE and Masson staining. Further more, observing the ultrastructure of lung tissue by transmission electron microscopy (TEM), the detection of JAK/STAT pathway related indicators including p-JAK1, p-STAT1, and SOCS3 with immunohistochemistry and SOCS3 with real-time PCR (RT-PCR) was performed.Results. Compared with the BLM group, the degree of alveolitis and fibrosis improved significantly, and the expression of p-JAK1 and p-STAT1 decreased; conversely, the expression of SOCS3 increased in the treatment group.Conclusion. IPF causes high expression of p-JAK1 and p-STAT1 and low expression of SOCS3. FOFB can play a role in the treatment of IPF via upregulating SOCS3 and downregulating p-JAK1 and p-STAT1.