文献类型：期刊文献

中文题名：抑瘤素M促进小鼠诱导多能干细胞向心肌分化

英文题名：Oncostatin M promotes the differentiation of murine induced pluripotent stem cells into cardiomyocytes

作者：王记[1];陆玉琴[1];徐鑫[1];纪召娟[1]

第一作者：王记

机构：[1]甘肃中医药大学附属医院心血管科,甘肃兰州730020

第一机构：甘肃中医药大学第二附属医院

年份：2017

卷号：37

期号：10

起止页码：1401

中文期刊名：基础医学与临床

外文期刊名：Basic and Clinical Medicine

收录：CSTPCD;;CSCD:【CSCD_E2017_2018】；

基金：甘肃省科技厅自然科学基金(1508RJZA044)

语种：中文

中文关键词：抑瘤素M;小鼠诱导多能干细胞;心肌细胞;分化

外文关键词：oncostatin M; miPSCs; myocardial cell; differentiation

摘要：目的以小鼠诱导多能干细胞(mi PSCs)为模型探讨抑瘤素M(OSM)在心肌细胞分化中的作用。方法用拟胚体分化法,在分化前中期(0~6 d)加入OSM培养分化15 d,实时荧光定量PCR、Western blot与免疫荧光染色法检测心肌细胞特异性标志物。结果 OSM诱导mi PSCs分化为心肌细胞的最佳浓度为20 pmol/L,此时心肌肌钙蛋白T(cTnT)表达为对照组的7倍(P<0.05);诱导组心肌细胞特异性基因和蛋白全面高于对照组(P<0.05);心肌特异性标志物cTnT染色显阳性,且与对照组相比,诱导组cTnT阳性细胞比率明显增高(P<0.05)。结论抑瘤素M促进了mi PSCs向心肌细胞的高效分化,为干细胞的心肌分化提供了一种有效的分化策略。
Objective To investigate the effects of oncostatin M（ OSM） on differentiation of murine induced pluripotent stem cells（ mi PSCs） into cardiomyocytes in vitro. Methods Using the embryoid bodies differentiation method,OSM was added during early-phase and midphase（ day 0-6） of mi PSCs differentiation for 15 days. Semi-quantitative RT-PCR,Western blot and immunofluorescence staining were used to detect the expression levels of myocardial cells-specific markers. Results The best concentration of OSM to induce the cardiac differentiation of mi PSCs was 20 pmol/L and cardiac troponin-T（ cTnT） was expressed 7-fold higher in OSM-treated compared with vehicle control-treated embryoid bodies at day 15 post-differentiation. Cardiac-specific gene and protein expression of cTnT and Gata4 demonstrated that the OSM-treated embryoid bodies expressed cardiac-specific gene（ Tnnt2,Nkx2. 5,MLC2 a,MLC2v,GATA4 and GATA6） and protein（ c Tn I,Gata4） at levels that were higher than those of the vehicle-control group after induced for 15 days. Furthermore,Immunostaining of the cardiac-specific protein markers cTnT showed that the mi PSCs expressed cTnT across all groups,and exhibited a markedly increased of cTnT-positive cells in OSM-treated cells. Conclusions OSM promotes the differentiation of murine induced pluripotent stem cells into cardiomyocytes,and it could serve as a valuable strategy for myocardiac differentiation of mi PSCs in vitro.